pntB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pntB

Mnemonic

Pyridine nucleotide transhydrogenase

Synonyms

ECK1597, b1602, JW1594[1], JW1594

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

36.06 minutes 

MG1655: 1674384..1672996
<gbrowseImage> name=NC_000913:1672996..1674384 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1653555..1652167
<gbrowseImage> name=NC_012967:1652167..1653555 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1565055..1566443
<gbrowseImage> name=NC_012759:1565055..1566443 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1678074..1676686
<gbrowseImage> name=NC_007779:1676686..1678074 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1765345..1763957
<gbrowseImage> name=NC_010473:1763957..1765345 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1672996

Edman degradation

PMID:3525165[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpntB (Keio:JW1594)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9355[4]

pntB::Tn5KAN-2 (FB20378)

Insertion at nt 862 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20378

contains pKD46

pntB::Tn5KAN-2 (FB20379)

Insertion at nt 862 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20379

does not contain pKD46

ΔpntB750::kan

PMID:16738554[3]

CGSC:102392


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1594

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCTGGAGGATTAGTTACAGC

Primer 2:CCaAGAGCTTTCAGGATTGCATC

E3F2

Kohara Phage

Genobase

PMID:3038334[7]

11H12

Kohara Phage

Genobase

PMID:3038334[7]

6A3

Kohara Phage

Genobase

PMID:3038334[7]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[4]

est. P1 cotransduction: % [8]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[4]

est. P1 cotransduction: % [8]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10745

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10745

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000736

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946144

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0738

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005352

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Clarke, DM et al. (1986) Nucleotide sequence of the pntA and pntB genes encoding the pyridine nucleotide transhydrogenase of Escherichia coli. Eur. J. Biochem. 158 647-53 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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