pmrD:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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Standard name

pmrD

Mnemonic

Polymyxin B resistance

Synonyms

ECK2253, b2259, JW2254[1], JW2254

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

51.11 minutes 

MG1655: 2371560..2371294
<gbrowseImage> name=NC_000913:2371294..2371560 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2320051..2319785
<gbrowseImage> name=NC_012967:2319785..2320051 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2257099..2257365
<gbrowseImage> name=NC_012759:2257099..2257365 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2378208..2377942
<gbrowseImage> name=NC_007779:2377942..2378208 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2462548..2462282
<gbrowseImage> name=NC_010473:2462282..2462548 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpmrD (Keio:JW2254)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9817[3]

pmrDL19I

L19I

(in strain: ECOR 23, ECOR 32, ECOR 38, ECOR 39, ECOR 40, ECOR 41, ECOR 51, ECOR 52, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65 and ECOR 66)

Strain variation; seeded from UniProt:P37590

pmrDM20K

M20K

(in strain: ECOR 46, ECOR 49 and O2:HN / ECOR 50 / P97 / UPEC)

Strain variation; seeded from UniProt:P37590

pmrDV83A

V83A

(in strain: ECOR 27, ECOR 33, ECOR 38, ECOR 39, ECOR 41, ECOR 44, ECOR 45, ECOR 47, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 63, ECOR 64, ECOR 65, ECOR 66 and ECOR 69)

Strain variation; seeded from UniProt:P37590

pmrDA84E

A84E

(in strain: ECOR 28 and ECOR 30)

Strain variation; seeded from UniProt:P37590

pmrDA27T

A27T

(in strain: ECOR 46, ECOR 48, ECOR 49 and O2:HN / ECOR 50 / P97 / UPEC)

Strain variation; seeded from UniProt:P37590

pmrDS71C

S71C

(in strain: ECOR 38, ECOR 39, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 61, ECOR 63, ECOR 64, ECOR 65 and ECOR 66)

Strain variation; seeded from UniProt:P37590

pmrDC81S

C81S

(in strain: ECOR 53, ECOR 59 and ECOR 60)

Strain variation; seeded from UniProt:P37590

pmrDK82T

K82T

(in strain: ECOR 26, ECOR 27, ECOR 29, ECOR 30, ECOR 33, ECOR 44, ECOR 45, ECOR 47, ECOR 58, ECOR 67, ECOR 68, ECOR 69 and ECOR 71)

Strain variation; seeded from UniProt:P37590

pmrDN11D

N11D

(in strain: ECOR 46, ECOR 48, ECOR 49 and O2:HN / ECOR 50 / P97 / UPEC)

Strain variation; seeded from UniProt:P37590

pmrDD14N

D14N

(in strain: ECOR 53, ECOR 59 and ECOR 60)

Strain variation; seeded from UniProt:P37590

ΔpmrD-741::kan

PMID:16738554[2]

CGSC:102384


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2254

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGAATGGCTGGTCAAAAAATC

Primer 2:CCtTGAGTTTTCCCTGCCACTTT

4B4

Kohara Phage

Genobase

PMID:3038334[5]

5H12

Kohara Phage

Genobase

PMID:3038334[5]

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[3]

est. P1 cotransduction: 34% [6]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[3]

est. P1 cotransduction: 52% [6]
Synonyms:zej-223::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7172

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12671

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003748

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945130

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2537

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007468

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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