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Standard Name	pitA
Gene Synonym(s)	ECK3478, b3493, JW3460, pit, G442^[1]^[2]
Product Desc.	PitA^[2]^[3] Low-affinity Pi transport protein^[4]
Product Synonyms(s)	phosphate transporter, low-affinity^[1], B3493^[2]^[1], Pit^[2]^[1], PitA^[2]^[1] , ECK3478, JW3460, pit, b3493
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pitA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	pitA
Mnemonic	Pi transport
Synonyms	ECK3478, b3493, JW3460, pit, G442^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	78.36 minutes	MG1655: 3635665..3637164 <gbrowseImage> name=NC_000913:3635665..3637164 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3568331..3569830 <gbrowseImage> name=NC_012967:3568331..3569830 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3524159..3525658 <gbrowseImage> name=NC_012759:3524159..3525658 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4002773..4001274 <gbrowseImage> name=NC_007779:4001274..4002773 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3733410..3734909 <gbrowseImage> name=NC_010473:3733410..3734909 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔpitA (Keio:JW3460)	deletion	deletion			PMID:16738554	Shigen CGSC10563^[5]
pitA10					PMID:4570598 PMID:4568763	CGSC:5633
pitA1						CGSC:15787
ΔpitA749::kan					PMID:16738554	CGSC:102374
pit in strain UR13	68 minutes		Resistant to	Resistant to arsenate	PMID:4919964	Strain: UR13	table 1
pit in strain 10B5	68 minutes		Auxotrophies	sn-glycerol-3-phosphate auxotroph	PMID:4919964	Strain: UR13	table 1
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Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3460	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTACATTTGTTTGCTGGCCT Primer 2:CCaAGGAACTGCAAGGAGAGCCA
12E4	Kohara Phage	Genobase	PMID:3038334
6A4	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: 14% ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12230	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12230	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002114	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948009	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2142	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011407	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PitA
Synonyms	phosphate transporter, low-affinity^[1], B3493^[2]^[1], Pit^[2]^[1], PitA^[2]^[1] , ECK3478, JW3460, pit, b3493
Product description	PitA^[2]^[3] Low-affinity Pi transport protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized to the periplasm with 10 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLHLFAGLDL HTGLLLLLAL AFVLFYEAIN GFHDTANAVA TVIYTRAMRS QLAVVMAAVF NFLGVLLGGL SVAYAIVHML PTDLLLNMGS SHGLAMVFSM LLAAIIWNLG TWYFGLPASS SHTLIGAIIG IGLTNALMTG TSVVDALNIP KVLSIFGSLI VSPIVGLVFA GGLIFLLRRY WSGTKKRARI HLTPAEREKK DGKKKPPFWT RIALILSAIG VAFSHGANDG QKGIGLVMLV LIGVAPAGFV VNMNATGYEI TRTRDAINNV EAYFEQHPAL LKQATGADQL VPAPEAGATQ PAEFHCHPSN TINALNRLKG MLTTDVESYD KLSLDQRSQM RRIMLCVSDT IDKVVKMPGV SADDQRLLKK LKSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVATTIG EKIGKKGMTY AQGMSAQMTA AVSIGLASYT GMPVSTTHVL SSSVAGTMVV DGGGLQRKTV TSILMAWVFT LPAAVLLSGG LYWLSLQFL
Length	499
Mol. Wt	53.39 kDa
pI	9.9 (calculated)
Extinction coefficient	61,880 - 62,130 (calc based on 12 Y, 8 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	30..488	PF01384 Phosphate transporter family		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pitA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131365	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948009	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011407	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFJ7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12230	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12230	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948009	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002114	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2142	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.50E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	3649	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	422	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	712	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pitA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3635645..3635685 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3493 (EcoliWiki Page)	NCBI GEO profiles for pitA
microarray	GenExpDB:b3493 (EcoliWiki Page)	Summary of data for pitA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3634942..3635291) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ16; Well:H3^[8]
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Notes

Accessions Related to pitA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12230	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2142	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3493
EcoGene	EcoGene:EG12230	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002114	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011407	Escherichia coli str. K-12 substr. MG1655
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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000019350 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G26570 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000010384 (score: 1.000; bootstrap: 100%) ENSBTAP00000039547 (score: 0.503)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00028227 (score: 1.000; bootstrap: 100%) WBGene00038466 (score: 0.317) WBGene00038370 (score: 0.297) WBGene00039864 (score: 0.196) WBGene00038578 (score: 0.143)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00016739 (score: 1.000; bootstrap: 100%) WBGene00017312 (score: 0.321) WBGene00015055 (score: 0.310) WBGene00015054 (score: 0.300) WBGene00012285 (score: 0.182) WBGene00007141 (score: 0.152)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000010724 (score: 1.000; bootstrap: 100%) ENSCAFP00000008202 (score: 0.509)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000016673 (score: 1.000; bootstrap: 100%) ENSCINP00000011191 (score: 0.460)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000016499 (score: 1.000; bootstrap: 100%) ENSGALP00000022289 (score: 0.204)	From Inparanoid:20070104
Homo sapiens	ENSP00000272542 (score: 1.000; bootstrap: 100%) ENSP00000340465 (score: 0.513)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000017371 (score: 1.000; bootstrap: 100%) ENSMODP00000012737 (score: 0.499)	From Inparanoid:20070104
Mus musculus	MGI:97851 (score: 1.000; bootstrap: 100%) MGI:108392 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ZH87 (score: 1.000; bootstrap: 100%) Q6ZH86 (score: 1.000; bootstrap: 100%) Q7YME0 (score: 0.857)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000021186 (score: 1.000; bootstrap: 100%) ENSPTRP00000034606 (score: 0.513)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000025294 (score: 1.000; bootstrap: 100%) ENSRNOP00000026418 (score: 0.166)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000173547 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000136265 (score: 0.486) NEWSINFRUP00000181415 (score: 0.457)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00029616001 (score: 1.000; bootstrap: 100%) GSTENP00017332001 (score: 0.446) GSTENP00006373001 (score: 0.396)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000029761 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	PITA	From SHIGELLACYC
E. coli O157	PITA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01384 Phosphate transporter family
EcoCyc	EcoCyc:EG12230	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12230	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002114	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2142	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011407	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

pitA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to pitA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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