phoH:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

phoH

Mnemonic

Phosphate

Synonyms

ECK1010, b1020, JW1005, psiH[1], psiH

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

23.37 minutes 

MG1655: 1084215..1085279
<gbrowseImage> name=NC_000913:1084215..1085279 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1101277..1102341
<gbrowseImage> name=NC_012967:1101277..1102341 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 987183..988247
<gbrowseImage> name=NC_012759:987183..988247 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1085414..1086478
<gbrowseImage> name=NC_007779:1085414..1086478 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1138143..1139207
<gbrowseImage> name=NC_010473:1138143..1139207 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1084215

Edman degradation

PMID:8444794[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔphoH (Keio:JW1005)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8986[4]

phoH::Tn5KAN-2 (FB20086)

Insertion at nt 606 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20086

does not contain pKD46

ΔphoH766::kan

PMID:16738554[3]

CGSC:102356


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1005

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGTAACGTCCTGTACTGGACA

Primer 2:CCACTGTAGGCATGCAGCGTACG

1E12

Kohara Phage

Genobase

PMID:3038334[7]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[4]

est. P1 cotransduction: 45% [8]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[4]

est. P1 cotransduction: 1% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11734

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11734

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001673

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948010

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1685

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003458

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kim, SK et al. (1993) Molecular analysis of the phoH gene, belonging to the phosphate regulon in Escherichia coli. J. Bacteriol. 175 1316-24 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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