phoE:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

phoE

Mnemonic

Phosphate

Synonyms

ECK0242, b0241, JW0231, ompE[1], ompE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.57 minutes 

MG1655: 259324..258269
<gbrowseImage> name=NC_000913:258269..259324 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 264592..263555
<gbrowseImage> name=NC_012967:263555..264592 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 258268..259323
<gbrowseImage> name=NC_012759:258268..259323 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 259324..258269
<gbrowseImage> name=NC_007779:258269..259324 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 233428..232373
<gbrowseImage> name=NC_010473:232373..233428 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

258272

Edman degradation

PMID:337109[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

phoE(del) (Keio:JW0231)

deletion

deletion

PMID:16738554[3]

Shigen

CGSC8466[4]

phoEF351Y,N,S,V

F351Y,N,S,V

Less resistant to trypsin

seeded from UniProt:P02932

phoE759(del)::kan

PMID:16738554[3]

CGSC:102354

phoE(del)

Resistant to

Increased resistance to acid, table 9.

PMID:1314797[5]

Experimental strain: 1157

phoE+

Sensitivity to

Increased acid sensitivity, table 9.

PMID:1314797[5]

1157-4


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0231

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAAAGAGCACTCTGGCATT

Primer 2:CCAAACTGATACGTCATGCCAAC

8F9

Kohara Phage

Genobase

PMID:3038334[7]

8G4

Kohara Phage

Genobase

PMID:3038334[7]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[4]

est. P1 cotransduction: 37% [8]
Synonyms:zae-502::Tn10

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[4]

est. P1 cotransduction: 95% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10729

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10729

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944926

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000720

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0722

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000823

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Henning, U et al. (1977) Major proteins of the outer cell envelope membrane of Escherichia coli K-12: multiple species of protein I. Mol. Gen. Genet. 154 293-8 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Rowbury, RJ et al. (1992) The PhoE porin and transmission of the chemical stimulus for induction of acid resistance (acid habituation) in Escherichia coli. J. Appl. Bacteriol. 72 233-43 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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