pheS:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
pheS |
|---|---|
| Mnemonic |
Phenylalanine |
| Synonyms |
ECK1712, b1714, JW5277, phe-act[1], phe-act |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
38.71 minutes |
MG1655: 1796966..1795983 |
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NC_012967: 1776209..1775226 |
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NC_012759: 1688042..1689025 |
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W3110 |
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W3110: 1800656..1799673 |
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DH10B: 1887537..1886554 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
pheSG98D |
G98D |
(in thermosensitive mutant pheS5; might cause subunit disaggregation due to electrostatic repulsion) |
Strain variation; seeded from UniProt:P08312 | ||||
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pheSG191D |
G191D |
(decreased affinity for Phe) |
Strain variation; seeded from UniProt:P08312 | ||||
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pheS11 |
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pheS5(ts) |
temperature sensitive |
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pheS in strain PFP-10 |
Resistant to |
Resistant to Gluoro(4)phenylalanine |
Strain: PFP-10 |
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pheS294 |
Serine to Alanine at position 294 |
Resistant to |
Resistant to Fluoro(4)phenylalanine |
Strain: AB1360-12 |
table 3 | ||
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pheS in strain KB1360 |
Resistant to |
Resistance to Fluoro(4)phenyalanine |
Strain: KB1360 |
table 1 | |||
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pheS in strain 6C5 |
Resistant to |
Thymineless death resistance in thyA. Under the condition of 42C. |
Strain: 6C5 |
Figure 4 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5277 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCACATCTCGCAGAACTGGT Primer 2:CCTTTAAACTGTTTGAGGAAACG | |
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Kohara Phage |
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Kohara Phage |
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zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: 50% [10] | |
|
Linked marker |
est. P1 cotransduction: 22% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Kast, P et al. (1992) Identification of the pheS5 mutation, which causes thermosensitivity of Escherichia coli mutant NP37. J. Bacteriol. 174 1686-9 PubMed
- ↑ FANGMAN, WL & NEIDHARDT, FC (1964) DEMONSTRATION OF AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE IN A MUTANT OF ESCHERICHIA COLI. J. Biol. Chem. 239 1839-43 PubMed
- ↑ Kast, P & Hennecke, H (1991) Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations. J. Mol. Biol. 222 99-124 PubMed
- ↑ Borg-Olivier, SA et al. (1987) Defective regulation of the phenylalanine biosynthetic operon in mutants of the phenylalanyl-tRNA synthetase operon. J. Bacteriol. 169 1949-53 PubMed
- ↑ Kaplan, S & Anderson, D (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J. Bacteriol. 95 991-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
