PMID:4868365
| Citation |
Kaplan, S and Anderson, D (1968) Selection of temperature-sensitive activating enzyme mutants in Escherichia coli. J. Bacteriol. 95:991-7 |
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| Abstract |
A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed. |
| Links | |
| Keywords |
Amino Acids/metabolism; Bacterial Proteins/biosynthesis; Carbon Isotopes; Escherichia coli/enzymology; Ligases/metabolism; Molecular Biology; Mutation; Proline/metabolism; RNA, Bacterial/biosynthesis; Temperature; Thymine/metabolism; Tritium; Uracil/metabolism |
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