PMID:3032903

From EcoliWiki
Jump to: navigation, search
Citation

Borg-Olivier, SA, Tarlinton, D and Brown, KD (1987) Defective regulation of the phenylalanine biosynthetic operon in mutants of the phenylalanyl-tRNA synthetase operon. J. Bacteriol. 169:1949-53

Abstract

Among mutants of Escherichia coli resistant to p-fluorophenylalanine (PFP) were some with constitutive expression of the phenylalanine biosynthetic operon (the pheA operon). This operon is repressed in the wild type by phenylalanine. The mutation in three of these mutants mapped in the aroH-aroD region of the E. coli chromosome at 37 min. A plasmid bearing wild-type DNA from this region restored p-fluorophenylalanine sensitivity and wild-type repression of the pheA operon. Analysis of subclones of this plasmid and comparison of its restriction map with published maps indicated that the mutations affecting regulation of the pheA operon lie in the structural genes for phenylalanyl-tRNA synthetase, pheST, probably in pheS. Thus, the pheST operon has a role in the regulation of phenylalanine biosynthesis, the most likely being that wild-type phenylalanyl-tRNA synthetase maintains a sufficient intracellular concentration of Phe-tRNA(Phe) for attenuation of the pheA operon in the presence of phenylalanine. A revised gene order for the 37-min region of the chromosome is reported. Read clockwise, the order is aroD, aroH, pheT, and pheS.

Links

PubMed PMC212056

Keywords

Amino Acyl-tRNA Synthetases/genetics; Chorismate Mutase/genetics; Chromosome Mapping; Cloning, Molecular; DNA Restriction Enzymes/diagnostic use; Escherichia coli/genetics; Gene Expression Regulation; Genes; Genes, Bacterial; Operon; Phenylalanine/biosynthesis; Phenylalanine/genetics; Phenylalanine-tRNA Ligase/genetics; Prephenate Dehydratase/genetics

Significance

You can help EcoliWiki by summarizing why this paper is useful

Useful Materials and Methods

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Annotations

<annotationlinks/>

EcoliWiki Links

Add links to pages that link here (e.g. gene, product, method pages)

See also

References

See Help:References for how to manage references in EcoliWiki.