nuoN:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoN |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2270, b2276, JW2271[1], JW2271 |
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Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.47 minutes |
MG1655: 2389347..2388070 |
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NC_012967: 2334849..2333572 |
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NC_012759: 2273875..2275332 |
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|
W3110 |
|
W3110: 2395995..2394718 |
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|
DH10B: 2481112..2479835 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔnuoN (Keio:JW2271) |
deletion |
deletion |
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|
nuoNM1H |
M1H |
Shows 20% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK158C |
K158C |
Shows 50% of the wild-type rate of deamino-NADH oxidase. Inhibited by 30-50% upon addition of 0.25 mM of decylubiquinone |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK217C |
K217C |
Loss of activity |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK217R |
K217R |
Shows 40% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
nuoNH224K |
H224K |
Shows 40% of the wild-type rate of deamino-NADH oxidase. Inhibited by 20-30% upon addition of 0.25 mM of decylubiquinone |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK247C |
K247C |
Shows 7% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
nuoNG391S |
G391S |
Shows 90% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK395C |
K395C |
Shows 5% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
nuoNK395R |
K395R |
Shows 30% of the wild-type rate of deamino-NADH oxidase |
seeded from UniProt:P0AFF0 | ||||
|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Dextrin |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire D-Galactose |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire D-Mannitol |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose-1-phosphate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire a-D-Glucose-6-phosphate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire DL-a-Glycerol |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire D-Galacturonate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire D-Glucuronate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire DL-Lactate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Inosine |
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|
ΔnuoN::kan |
deletion |
Biolog:respiration |
unable to respire Uridine |
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|
ΔnuoN758::kan |
| ||||||
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Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2271 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGACGTTACGCCGCTGATGCG Primer 2:CCCATCAGCGGCATTGCCAAACG | |
|
Kohara Phage |
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|
Kohara Phage |
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|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 14% [7] | ||
|
Linked marker |
est. P1 cotransduction: 96% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 4.24 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 6.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).