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Product(s) |

Expression|

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Standard Name	nrdF
Gene Synonym(s)	ECK2670, b2676, JW2651^[1], JW2651, ygaD
Product Desc.	NrdF^[2]^[3]; Component of ribonucleoside-diphosphate reductase 2, β subunit dimer^[2]^[3]; ribonucleoside-diphosphate reductase 2^[2]^[3] Ribonucleoside diphosphate reductase 2, subunit beta, class I^[4]
Product Synonyms(s)	ribonucleoside-diphosphate reductase 2, beta subunit, ferritin-like^[1], B2676^[2]^[1], NrdF^[2]^[1] , ECK2670, JW2651, ygaD, b2676
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nrdHIEF^[2], nrdEF
Regulation/Activity
Quick Links
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Notes

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nrdF is a nonessential gene.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nrdF
Mnemonic	Nucleotide reductase
Synonyms	ECK2670, b2676, JW2651^[1], JW2651, ygaD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.38 minutes	MG1655: 2801524..2802483 <gbrowseImage> name=NC_000913:2801524..2802483 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2698254..2699213 <gbrowseImage> name=NC_012967:2698254..2699213 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2687336..2688295 <gbrowseImage> name=NC_012759:2687336..2688295 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2802158..2803117 <gbrowseImage> name=NC_007779:2802158..2803117 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2893289..2894248 <gbrowseImage> name=NC_010473:2893289..2894248 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔnrdF (Keio:JW2651)	deletion	deletion	PMID:16738554	Shigen CGSC10090^[5]
ΔnrdF757::kan			PMID:16738554	CGSC:102108
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2651	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACTCTCACGTATCAGCGC Primer 2:CCGAAATTCCAGTCTTCATCTTC
10H9	Kohara Phage	Genobase	PMID:3038334
8G10	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[5]	est. P1 cotransduction: 3% ^[6]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 41% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12381	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12381	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002248	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947149	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2283	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008808	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NrdF
Synonyms	ribonucleoside-diphosphate reductase 2, beta subunit, ferritin-like^[1], B2676^[2]^[1], NrdF^[2]^[1] , ECK2670, JW2651, ygaD, b2676
Product description	NrdF^[2]^[3]; Component of ribonucleoside-diphosphate reductase 2, β subunit dimer^[2]^[3]; ribonucleoside-diphosphate reductase 2^[2]^[3] Ribonucleoside diphosphate reductase 2, subunit beta, class I^[4]
EC number (for enzymes)	1.17.4.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	PMID:7809142	ISA: Inferred from Sequence Alignment	UniProtKB:Q08698	C		complete
Contributes to	GO:0004748	ribonucleoside-diphosphate reductase activity	PMID:7809142	IGI: Inferred from Genetic Interaction	EcoliWiki:nrdA\|EcoliWiki:nrdB	F		complete
	GO:0005971	ribonucleoside-diphosphate reductase complex	PMID:7809142	IGI: Inferred from Genetic Interaction	EcoliWiki:nrdA\|EcoliWiki:nrdB	C		complete
	GO:0004748	ribonucleoside-diphosphate reductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000358	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009263	deoxyribonucleotide biosynthetic process	PMID:7809142	IGI: Inferred from Genetic Interaction	EcoliWiki:nrdA\|EcoliWiki:nrdB	P		complete
	GO:0004748	ribonucleoside-diphosphate reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.17.4.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	PMID:7809142	ISA: Inferred from Sequence Alignment	UniProtKB:Q08698	F		complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009186	deoxyribonucleoside diphosphate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000358	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR009078	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012348	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046914	transition metal ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR009078	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000358	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR009078	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012348	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of ribonucleoside-diphosphate reductase 2, β subunit dimer	could be indirect
Protein	pepD		PMID:16606699	Experiment(s):EBI-1143651
Protein	rplQ		PMID:16606699	Experiment(s):EBI-1143651
Protein	ykgF		PMID:16606699	Experiment(s):EBI-1143651
Protein	rlmB		PMID:16606699	Experiment(s):EBI-1143651
Protein	cysM		PMID:16606699	Experiment(s):EBI-1143651
Protein	Subunits of ribonucleoside-diphosphate reductase 2	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKLSRISAIN WNKISDDKDL EVWNRLTSNF WLPEKVPLSN DIPAWQTLTV VEQQLTMRVF TGLTLLDTLQ NVIGAPSLMP DALTPHEEAV LSNISFMEAV HARSYSSIFS TLCQTKDVDA AYAWSEENAP LQRKAQIIQQ HYRGDDPLKK KIASVFLESF LFYSGFWLPM YFSSRGKLTN TADLIRLIIR DEAVHGYYIG YKYQKNMEKI SLGQREELKS FAFDLLLELY DNELQYTDEL YAETPWADDV KAFLCYNANK ALMNLGYEPL FPAEMAEVNP AILAALSPNA DENHDFFSGS GSSYVMGKAV ETEDEDWNF
Length	319
Mol. Wt	36.444 kDa
pI	4.5 (calculated)
Extinction coefficient	66,350 - 66,600 (calc based on 15 Y, 8 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..281	PF00268 Ribonucleotide reductase, small chain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nrdF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130590	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947149	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008808	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37146	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12381	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12381	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947149	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002248	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2283	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	88	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	42	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	28^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nrdHIEF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2801504..2801544 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2676 (EcoliWiki Page)	NCBI GEO profiles for nrdF
microarray	GenExpDB:b2676 (EcoliWiki Page)	Summary of data for nrdF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nrdF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12381	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2283	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2676
EcoGene	EcoGene:EG12381	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002248	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008808	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	NRDF	From SHIGELLACYC
E. coli O157	NRDF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:47240
Panther (EcoliWiki Page)	PTHR23409:SF5
Pfam (EcoliWiki Page)	PF00268 Ribonucleotide reductase, small chain
EcoCyc	EcoCyc:EG12381	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12381	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002248	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2283	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008808	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nrdF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nrdF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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