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Standard Name	nemA
Gene Synonym(s)	ECK1646, b1650, JW1642, ydhN^[1], ydhN
Product Desc.	N-ethylmaleimide reductase, FMN-linked^[2]^[3] N-Ethylmaleimide reductase^[4]
Product Synonyms(s)	N-ethylmaleimide reductase, FMN-linked^[1], B1650^[2]^[1], YdhN^[2]^[1], NemA^[2]^[1] , ECK1646, JW1642, ydhN, b1650
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s):
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nemA
Mnemonic	N-Ethylmaleimide
Synonyms	ECK1646, b1650, JW1642, ydhN^[1], ydhN
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	37.17 minutes, 37.17 minutes	MG1655: 1724683..1725780 <gbrowseImage> name=NC_000913:1724683..1725780 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1703853..1704950 <gbrowseImage> name=NC_012967:1703853..1704950 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1616742..1617839 <gbrowseImage> name=NC_012759:1616742..1617839 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1728373..1729470 <gbrowseImage> name=NC_007779:1728373..1729470 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1815254..1816351 <gbrowseImage> name=NC_010473:1815254..1816351 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔnemA (Keio:JW1642)	deletion	deletion	PMID:16738554	Shigen CGSC9397^[5]
ΔnemA728::kan			PMID:16738554	CGSC:102046
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1642	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCATCTGAAAAACTGTATTC Primer 2:CCtAACGTCGGGTAATCGGTATA
6F11	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6890	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003479	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946164	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3316	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005516	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NemA
Synonyms	N-ethylmaleimide reductase, FMN-linked^[1], B1650^[2]^[1], YdhN^[2]^[1], NemA^[2]^[1] , ECK1646, JW1642, ydhN, b1650
Product description	N-ethylmaleimide reductase, FMN-linked^[2]^[3] N-Ethylmaleimide reductase^[4]
EC number (for enzymes)	1.-.-.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0006805	xenobiotic metabolic process	PMID:9013822	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc (v14.0)	complete
	GO:0008748	N-ethylmaleimide reductase activity	PMID:9013822	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0010181	FMN binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001155	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	sapF	PMID:16606699	Experiment(s):EBI-1140366
Protein	ribB	PMID:16606699	Experiment(s):EBI-1140366
Protein	slyD	PMID:16606699	Experiment(s):EBI-1140366
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSSEKLYSPL KVGAITAANR IFMAPLTRLR SIEPGDIPTP LMAEYYRQRA SAGLIISEAT QISAQAKGYA GAPGIHSPEQ IAAWKKITAG VHAENGHMAV QLWHTGRISH ASLQPGGQAP VAPSALSAGT RTSLRDENGQ AIRVETSMPR ALELEEIPGI VNDFRQAIAN AREAGFDLVE LHSAHGYLLH QFLSPSSNHR TDQYGGSVEN RARLVLEVVD AGIEEWGADR IGIRVSPIGT FQNTDNGPNE EADALYLIEQ LGKRGIAYLH MSEPDWAGGE PYTDAFREKV RARFHGPIIG AGAYTVEKAE TLIGKGLIDA VAFGRDWIAN PDLVARLQRK AELNPQRAES FYGGGAEGYT DYPTL
Length	365
Mol. Wt	39.516 kDa
pI	6.2 (calculated)
Extinction coefficient	46,870 (calc based on 13 Y, 5 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..344	PF00724 NADH:flavin oxidoreductase / NADH oxidase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nemA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129608	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946164	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005516	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77258	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6890	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13546	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946164	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003479	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3316	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	4.05E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	514	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	220	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	267	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1724663..1724703 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1650 (EcoliWiki Page)	NCBI GEO profiles for nemA
microarray	GenExpDB:b1650 (EcoliWiki Page)	Summary of data for nemA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nemA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6890	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3316	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1650
EcoGene	EcoGene:EG13546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003479	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005516	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G76690 (score: 1.000; bootstrap: 100%) AT1G76680 (score: 0.827) AT1G09400 (score: 0.457) AT1G17990 (score: 0.235) AT1G18020 (score: 0.235) AT2G06050 (score: 0.227)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00030867 (score: 1.000; bootstrap: 100%) WBGene00038198 (score: 0.676) WBGene00024591 (score: 0.632) WBGene00024604 (score: 0.582) WBGene00039534 (score: 0.265) WBGene00039533 (score: 0.232)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00021066 (score: 1.000; bootstrap: 100%) WBGene00017537 (score: 1.000; bootstrap: 100%) WBGene00020394 (score: 0.917) WBGene00017536 (score: 0.650) WBGene00022267 (score: 0.649) WBGene00010148 (score: 0.594) WBGene00022813 (score: 0.268) WBGene00022814 (score: 0.253)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0205787 (score: 1.000; bootstrap: 100%) DDB0187621 (score: 0.596) DDB0187193 (score: 0.434) DDB0191789 (score: 0.419) DDB0204331 (score: 0.414) DDB0203608 (score: 0.353)	From Inparanoid:20070104
Oryza gramene	Q69TH4 (score: 1.000; bootstrap: 100%) Q8H9F1 (score: 0.922) Q84QK0 (score: 0.922) Q69TH8 (score: 0.770) Q69TH6 (score: 0.735) Q49HE4 (score: 0.721) Q69TI2 (score: 0.699) Q69TI0 (score: 0.699) Q49HE3 (score: 0.683) Q49HE2 (score: 0.651) Q49HE1 (score: 0.517) Q69TH2 (score: 0.487) Q49HE0 (score: 0.475) Q49HD9 (score: 0.457) Q6YU32 (score: 0.443) Q84QJ9 (score: 0.387) Q6Z965 (score: 0.281) Q49HD7 (score: 0.263) Q49HD8 (score: 0.255)	From Inparanoid:20070104
Saccharomyces cerevisiae	YPL171C (score: 1.000; bootstrap: 100%) YHR179W (score: 0.773)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC5H104 (score: 1.000; bootstrap: 100%) SPAC5H100 (score: 0.479)	From Inparanoid:20070104
Shigella flexneri	NEMA	From SHIGELLACYC
E. coli O157	NEMA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00724 NADH:flavin oxidoreductase / NADH oxidase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:51395
Panther (EcoliWiki Page)	PTHR22893:SF18
EcoCyc	EcoCyc:G6890	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003479	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3316	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005516	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nemA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nemA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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