mukF:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
mukF |
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Gene Synonym(s) |
ECK0913, b0922, JW0905, kicB[1], kicB |
Product Desc. |
Ca2+-binding protein involved in chromosome partitioning[2][3]; Component of MukB-MukE-MukF complex[3] Chromosome condensin MukBEF, kleisin-like subunit[4] |
Product Synonyms(s) |
Involved in chromosome partioning, Ca2+ binding protein[1], B0922[2][1], KicB[2][1], MukF[2][1] , ECK0913, JW0905, kicB, b0922 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression |
transcription unit(s): smtA-mukFEB[2] |
Regulation/Activity | |
Quick Links | |
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Notes
Condensin is required for chromosome partitioning and compaction. Null mutants die above 30C and produce some anucleate cells at permissive temperatures.[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
mukF |
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Mnemonic |
Mukaku (anucleate) |
Synonyms |
ECK0913, b0922, JW0905, kicB[1], kicB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
20.98 minutes |
MG1655: 973542..974864 |
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NC_012967: 991411..992733 |
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NC_012759: 876510..877832 |
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W3110 |
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W3110: 974741..976063 |
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DH10B: 1027470..1028792 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
mukFL233P |
L233P |
Abolishes function |
seeded from UniProt:P60293 | ||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0905 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAGTGAATTTTCCCAGACAGT Primer 2:CCATATTTGTCGATGACATGCGC | |
Kohara Phage |
PMID:3038334 | ||
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 36% [6] | |
Linked marker |
est. P1 cotransduction: 33% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002061 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB2084 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003134 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
MukF |
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Synonyms |
Involved in chromosome partioning, Ca2+ binding protein[1], B0922[2][1], KicB[2][1], MukF[2][1] , ECK0913, JW0905, kicB, b0922 |
Product description |
Ca2+-binding protein involved in chromosome partitioning[2][3]; Component of MukB-MukE-MukF complex[3] Chromosome condensin MukBEF, kleisin-like subunit[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005509 |
calcium ion binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01803 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005509 |
calcium ion binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005582 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005509 |
calcium ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0106 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01803 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005582 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01803 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005582 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007049 |
cell cycle |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0131 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007059 |
chromosome segregation |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01803 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007059 |
chromosome segregation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005582 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0007059 |
chromosome segregation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0159 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009295 |
nucleoid |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0187 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0051301 |
cell division |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0132 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of MukB-MukE-MukF complex |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
cytoplasm |
From EcoCyc[3] |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MSEFSQTVPE LVAWARKNDF SISLPVDRLS FLLAVATLNG ERLDGEMSEG ELVDAFRHVS DAFEQTSETI GVRANNAIND MVRQRLLNRF TSEQAEGNAI YRLTPLGIGI TDYYIRQREF STLRLSMQLS IVAGELKRAA DAAEEGGDEF HWHRNVYAPL KYSVAEIFDS IDLTQRLMDE QQQQVKDDIA QLLNKDWRAA ISSCELLLSE TSGTLRELQD TLEAAGDKLQ ANLLRIQDAT MTHDDLHFVD RLVFDLQSKL DRIISWGQQS IDLWIGYDRH VHKFIRTAID MDKNRVFAQR LRQSVQTYFD EPWALTYANA DRLLDMRDEE MALRDEEVTG ELPEDLEYEE FNEIREQLAA IIEEQLAVYK TRQVPLDLGL VVREYLSQYP RARHFDVARI VIDQAVRLGV AQADFTGLPA KWQPINDYGA KVQAHVIDKY |
Length |
440 |
Mol. Wt |
50.579 kDa |
pI |
4.6 (calculated) |
Extinction coefficient |
59,360 - 59,485 (calc based on 14 Y, 7 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0003134 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120002061 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2084 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
11+/-0.069 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.040847201 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
633 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
204 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
426 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:973522..973562
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
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This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for mukF | |
microarray |
Summary of data for mukF from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to mukF Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2084 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002061 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003134 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
MUKF |
From SHIGELLACYC |
E. coli O157 |
MUKF |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG12165 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120002061 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB2084 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003134 |
Escherichia coli str. K-12 substr. MG1655 | |
edit table |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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