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Standard Name	mreD
Gene Synonym(s)	ECK3237, b3249, JW3218^[1], JW3218
Product Desc.	MreD^[2]^[3]; Component of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex^[3] Mecillinam resistance; rod shape-determining protein^[4]
Product Synonyms(s)	cell wall structural complex MreBCD transmembrane component MreD^[1], B3249^[2]^[1], MreD^[2]^[1] , ECK3237, JW3218, b3249
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mreBCD-yhdE-rng^[2]
Regulation/Activity
Quick Links
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Notes

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In operon with mreBC and the gene products form an essential membrane-bound complex responsible for cell morphology^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mreD
Mnemonic	Murein cluster e; mecillinam resistance
Synonyms	ECK3237, b3249, JW3218^[1], JW3218
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.2 minutes	MG1655: 3396897..3396409 <gbrowseImage> name=NC_000913:3396409..3396897 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3326766..3326278 <gbrowseImage> name=NC_012967:3326278..3326766 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3283557..3284045 <gbrowseImage> name=NC_012759:3283557..3284045 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3398730..3398242 <gbrowseImage> name=NC_007779:3398242..3398730 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3494642..3494154 <gbrowseImage> name=NC_010473:3494154..3494642 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
mre-129	Resistant to	Mecillinam resistance at 42C	PMID:2822655 & ^[5]	Strain: AT1325-678	table 2
mre-678	Cell Shape	Sphere shaped	PMID:2822655 & ^[6]	Strain: AT1325-678	table 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3218	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCGAGCTATCGTAGCCAGGG Primer 2:CCTTGCACTGCAAACTGCTGACG
21D3	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[7]	est. P1 cotransduction: 27% ^[8] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[7]	est. P1 cotransduction: 52% ^[8] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10610	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10610	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000603	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947756	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0605	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010656	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MreD
Synonyms	cell wall structural complex MreBCD transmembrane component MreD^[1], B3249^[2]^[1], MreD^[2]^[1] , ECK3237, JW3218, b3249
Product description	MreD^[2]^[3]; Component of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex^[3] Mecillinam resistance; rod shape-determining protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007227	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008360	regulation of cell shape	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0133	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007227	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of longitudinal peptidoglycan synthesis/chromosome segregation-directing complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized in the cytoplasm with 5 predicted transmembrane domains	Daley et al. (2005) ^[9]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MASYRSQGRW VIWLSFLIAL LLQIMPWPDN LIVFRPNWVL LILLYWILAL PHRVNVGTGF VMGAILDLIS GSTLGVRVLA MSIIAYLVAL KYQLFRNLAL WQQALVVMLL SLVVDIIVFW AEFLVINVSF RPEVFWSSVV NGVLWPWIFL LMRKVRQQFA VQ
Length	162
Mol. Wt	18.787 kDa
pI	11.0 (calculated)
Extinction coefficient	60,960 (calc based on 4 Y, 10 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..155	PF04093 rod shape-determining protein MreD		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mreD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131137	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947756	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010656	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABH4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10610	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10610	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947756	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000603	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0605	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	367	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	71	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	148	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mreBCD-yhdE-rng
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3396877..3396917 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3249 (EcoliWiki Page)	NCBI GEO profiles for mreD
microarray	GenExpDB:b3249 (EcoliWiki Page)	Summary of data for mreD from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mreD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10610	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0605	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3249
EcoGene	EcoGene:EG10610	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000603	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010656	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MRED	From SHIGELLACYC
E. coli O157	MRED	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04093 rod shape-determining protein MreD
EcoCyc	EcoCyc:EG10610	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10610	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000603	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0605	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010656	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
↑ Okada, Y., M. Eachi,K. Nagai, and M. Matsihashi. 1992. Chage of the quantity of penicillin-binding proteins and other cytoplasmic and membrane proteins by mutations of the cell shape-determination genes mreB mreC mreD of Escherichia coli. J. Gen. Appl. Microbiol. 38:157-167.
↑ ^7.0 ^7.1 CGSC: The Coli Genetics Stock Center
↑ ^8.0 ^8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

mreD:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mreD Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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