mnmE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
mnmE |
|---|---|
| Mnemonic |
Methyl-amino(N)-Methyl |
| Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
83.73 minutes |
MG1655: 3884851..3886215 |
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NC_012967: 3847189..3848553 |
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NC_012759: 3773184..3774548 |
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W3110 |
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W3110: 3753587..3752223 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
mnmED338N |
D338N |
Strong decrease in GTP binding. Does not affect hydrolase activity, but has 10-fold higher affinity for XTP than for GTP. Partially impairs tRNA modifying function |
seeded from UniProt:P25522 | ||||
|
thdF::Tn5KAN-I-SceI (FB21404) |
Insertion at nt 378 in Plus orientation |
contains pKD46 | |||||
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thdF::Tn5KAN-I-SceI (FB21405) |
Insertion at nt 378 in Plus orientation |
does not contain pKD46 | |||||
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mnmET251S |
T251S |
4-fold decrease in GTPase activity and 1.2-fold decrease of affinity |
seeded from UniProt:P25522 | ||||
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mnmER252A |
R252A |
7-fold decrease in GTPase activity and 6-fold increase of affinity |
seeded from UniProt:P25522 | ||||
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mnmER288A |
R288A |
1.7-fold decrease in GTPase activity and 1.5-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmEG249A |
G249A |
22-fold decrease in GTPase activity and 7-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmET250A |
T250A |
4-fold decrease in GTPase activity and 1.5-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmET250S |
T250S |
1.8-fold decrease in GTPase activity and 1.5-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmED270A |
D270A |
Does not affect GTP binding, but impairs hydrolase activity. Completely impairs tRNA modifying function |
seeded from UniProt:P25522 | ||||
|
mnmEL255D |
L255D |
1.5-fold decrease in affinity for GTP |
seeded from UniProt:P25522 | ||||
|
mnmER256A |
R256A |
2-fold decrease in GTPase activity and almost no change in affinity |
seeded from UniProt:P25522 | ||||
|
mnmEN226K |
N226K |
70-fold decrease in GTPase activity. 2-fold decrease of affinity for GTP |
seeded from UniProt:P25522 | ||||
|
mnmEG228A |
G228A |
Loss of GTP binding and hydrolase activity. Completely impairs tRNA modifying function |
seeded from UniProt:P25522 | ||||
|
mnmEC451S |
C451S |
No change in GTP binding and hydrolase activity. Does not affect association to the cell inner membrane. Completely impairs tRNA modifying function |
seeded from UniProt:P25522 | ||||
|
mnmER224A |
R224A |
1.5-fold decrease in GTPase activity and almost no change in affinity |
seeded from UniProt:P25522 | ||||
|
mnmET251A |
T251A |
92-fold decrease in GTPase activity and 59-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmER275A |
R275A |
6-fold decrease in GTPase activity and 1.9-fold increase of affinity |
seeded from UniProt:P25522 | ||||
|
mnmEE282A |
E282A |
1900-fold decrease in GTPase activity |
seeded from UniProt:P25522 | ||||
|
mnmEE282Q |
E282Q |
370-fold decrease in GTPase activity |
seeded from UniProt:P25522 | ||||
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mnmEN226A |
N226A |
100-fold decrease in GTPase activity. 5-fold decrease of affinity for GTP |
seeded from UniProt:P25522 | ||||
|
mnmER252K |
R252K |
2-fold decrease in GTPase activity and no change in affinity |
seeded from UniProt:P25522 | ||||
|
mnmED253A |
D253A |
9-fold decrease in GTPase activity and 13-fold increase of affinity |
seeded from UniProt:P25522 | ||||
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ΔtrmE737::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
Plasmid clone |
Status: Primer 1: Primer 2: | ||
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Kohara Phage |
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Kohara Phage |
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|
Kohara Phage |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
