mnmE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TrmE

Synonyms

GTPase[1], B3706[2][1], MnmE[2][1], TrmE[2][1], ThdF[2][1], O454[2][1] , ECK3699, JW3684, thdF, trmE, b3706

Product description

GTP-binding protein with a role in modification of tRNA[2][3]

5-methylaminomethyl-2-thiouridine modification at tRNA U34; cmnm(5) addition to tRNA-s(2)U34, forming (with subsequent or interdependent MnmG action) cmnm(5)s(2)U34-tRNA, an intermediate in mnm(5)s(2)U34-tRNA synthesis[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00379

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004520

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00379

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002917

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004520

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005225

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0630

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceE

PMID:15690043[5]

Experiment(s):EBI-890013, EBI-894976

Protein

rpsB

PMID:15690043[5]

Experiment(s):EBI-890013, EBI-894976

Protein

yliL

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

dcm

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

dsbA

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

hupA

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

lpdA

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

mdh

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rplC

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rplD

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rplM

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rplV

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rpmG

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rpsF

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rpsG

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rpsJ

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

rpsN

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

sapF

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

secA

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

pgaD

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

yidA

PMID:15690043[5]

Experiment(s):EBI-894976

Protein

dsbA

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

mdh

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

secA

PMID:19402753[6]

LCMS(ID Probability):99.0 MALDI(Z-score):14.201221

Protein

yidA

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

sapF

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

mnmG

PMID:19402753[6]

LCMS(ID Probability):99.0 MALDI(Z-score):27.178626

Protein

dcm

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

yliL

PMID:19402753[6]

LCMS(ID Probability):99.0

Protein

pgaD

PMID:19402753[6]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSDNDTIVAQ ATPPGRGGVG ILRISGFKAR EVAETVLGKL PKPRYADYLP FKDADGSVLD
QGIALWFPGP NSFTGEDVLE LQGHGGPVIL DLLLKRILTI PGLRIARPGE FSERAFLNDK
LDLAQAEAIA DLIDASSEQA ARSALNSLQG AFSARVNHLV EALTHLRIYV EAAIDFPDEE
IDFLSDGKIE AQLNDVIADL DAVRAEARQG SLLREGMKVV IAGRPNAGKS SLLNALAGRE
AAIVTDIAGT TRDVLREHIH IDGMPLHIID TAGLREASDE VERIGIERAW QEIEQADRVL
FMVDGTTTDA VDPAEIWPEF IARLPAKLPI TVVRNKADIT GETLGMSEVN GHALIRLSAR
TGEGVDVLRN HLKQSMGFDT NMEGGFLARR RHLQALEQAA EHLQQGKAQL LGAWAGELLA
EELRLAQQNL SEITGEFTSD DLLGRIFSSF CIGK
Length

454

Mol. Wt

49.232 kDa

pI

4.8 (calculated)

Extinction coefficient

26,470 - 26,595 (calc based on 3 Y, 4 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

228..336

PF01926 GTPase of unknown function

PMID:19920124[7]

Domain

6..120

PF10396 GTP-binding protein TrmE N-terminus

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mnmE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131574

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948222

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012123

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P25522

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10997

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10997

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948222

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000986

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0990

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 5.14 5.15 5.16 5.17 5.18 5.19 5.20 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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