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Gene |

Product(s) |

Expression|

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Standard Name	mngB
Gene Synonym(s)	ECK0720, b0732, JW0721, ybgG^[1], ybgG
Product Desc.	α-mannosidase^[2]^[3] alpha-Mannosidase, 2-O-alpha-mannosyl-D-glycerate catabolism^[4]
Product Synonyms(s)	alpha-mannosidase^[1], B0732^[2]^[1], YbgG^[2]^[1], MngB^[2]^[1] , ECK0720, JW0721, ybgG, b0732
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mngAB^[2]
Regulation/Activity
Quick Links
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Notes

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AFMB Structural Genomics target No. 109 (http://afmb.cnrs-mrs.fr/article171.html).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mngB
Mnemonic	ManNosyl Glycerate
Synonyms	ECK0720, b0732, JW0721, ybgG^[1], ybgG
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	16.54 minutes	MG1655: 767201..769834 <gbrowseImage> name=NC_000913:767201..769834 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 669961..672594 <gbrowseImage> name=NC_012759:669961..672594 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 768400..771033 <gbrowseImage> name=NC_007779:768400..771033 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 819793..822426 <gbrowseImage> name=NC_010473:819793..822426 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
mngB(del) (Keio:JW0721)	deletion	deletion	PMID:16738554	Shigen CGSC8789^[5]
mngB781(del)::kan			PMID:16738554	CGSC:101892
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0721	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGCAGTATCTCGCGTTCA Primer 2:CCGGCAAGCCGGTAACTGAACGT
18F11	Kohara Phage	Genobase	PMID:3038334
10G5	Kohara Phage	Genobase	PMID:3038334
asnB3057::Tn10	Linked marker	CAG18433 = CGSC7349^[5]	est. P1 cotransduction: 2% ^[6] Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.
nadA57::Tn10	Linked marker	CAG12147 = CGSC7351^[5]	est. P1 cotransduction: 65% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG13236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002433	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945359	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3025	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002495	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MngB
Synonyms	alpha-mannosidase^[1], B0732^[2]^[1], YbgG^[2]^[1], MngB^[2]^[1] , ECK0720, JW0721, ybgG, b0732
Product description	α-mannosidase^[2]^[3] alpha-Mannosidase, 2-O-alpha-mannosyl-D-glycerate catabolism^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011330	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015341	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004559	alpha-mannosidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000602	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000602	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011330	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006013	mannose metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011682	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015341	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015923	mannosidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011682	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016798	hydrolase activity, acting on glycosyl bonds	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0326	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004559	alpha-mannosidase activity	PMID:14645248	IMP: Inferred from Mutant Phenotype		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpmB	PMID:16606699	Experiment(s):EBI-1137840
Protein	sdhA	PMID:16606699	Experiment(s):EBI-1137840
Protein	fdnG	PMID:16606699	Experiment(s):EBI-1137840
Protein	groL	PMID:16606699	Experiment(s):EBI-1137840
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKAVSRVHIT PHMHWDREWY FTTEESRILL VNNMEEILCR LEQDNEYKYY VLDGQTAILE DYFAVKPENK DRVKKQVEAG KLIIGPWYTQ TDTTIVSAES IVRNLMYGMR DCLAFGEPMK IGYLPDSFGM SGQLPHIYNG FGITRTMFWR GCSERHGTDK TEFLWQSSDG SEVTAQVLPL GYAIGKYLPA DENGLRKRLD SYFDVLEKAS VTKEILLPNG HDQMPLQQNI FEVMDKLREI YPQRKFVMSR FEEVFEKIEA QRDNLATLKG EFIDGKYMRV HRTIGSTRMD IKIAHARIEN KIVNLLEPLA TLAWTLGFEY HHGLLEKMWK EILKNHAHDS IGCCCSDKVH REIVARFELA EDMADNLIRF YMRKIADNMP QSDADKLVLF NLMPWPREEV INTTVRLRAS QFNLRDDRGQ PVPYFIRHAR EIDPGLIDRQ IVHYGNYDPF MEFDIQINQI VPSMGYRTLY IEANQPGNVI AAKSDAEGIL ENAFWQIALN EDGSLQLVDK DSGVRYDRVL QIEESSDDGD EYDYSPAKEE WVITAANAKP QCDIIHEAWQ SRAVIRYDMA VPLNLSERSA RQSTGRVGVV LVVTLSHNSR RIDVDINLDN QADDHRLRVL VPTPFNTDSV LADTQFGSLT RPVNDSAMNN WQQEGWKEAP VPVWNMLNYV ALQEGRNGMA VFSEGLREFE VIGEEKKTFA ITLLRGVGLL GKEDLLLRPG RPSGIKMPVP DSQLRGLLSC RLSLLSYTGT PTAAGVAQQA RAWLTPVQCY NKIPWDVMKL NKAGFNVPES YSLLKMPPVG CLISALKKAE DRQEVILRLF NPAESATCDA TVAFSREVIS CSETMMDEHI TTEENQGSNL SGPFLPGQSR TFSYRLA
Length	877
Mol. Wt	100.016 kDa
pI	5.6 (calculated)
Extinction coefficient	132,700 - 134,200 (calc based on 30 Y, 16 W, and 12 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	6..274	PF01074 Glycosyl hydrolases family 38 N-terminal domain	PMID:19920124
Domain	385..862	PF07748 Glycosyl hydrolases family 38 C-terminal domain	PMID:19920124
Domain	279..357	PF09261 Alpha mannosidase, middle domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mngB taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128707	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945359	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002495	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P54746	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG13236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13236	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945359	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002433	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3025	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	18	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	12	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mngAB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:767181..767221 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0732 (EcoliWiki Page)	NCBI GEO profiles for mngB
microarray	GenExpDB:b0732 (EcoliWiki Page)	Summary of data for mngB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mngB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG13236	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3025	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0732
EcoGene	EcoGene:EG13236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002433	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002495	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Ciona intestinalis	ENSCINP00000012206 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0231611 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0027611 (score: 1.000; bootstrap: 100%) FBgn0032253 (score: 0.398) FBgn0032068 (score: 0.397) FBgn0032067 (score: 0.386) FBgn0032069 (score: 0.383) FBgn0032066 (score: 0.379) FBgn0011740 (score: 0.104) FBgn0026616 (score: 0.081)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000129282 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00015999001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YBGG	From SHIGELLACYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01074 Glycosyl hydrolases family 38 N-terminal domain
Panther (EcoliWiki Page)	PTHR11607:SF2
Superfamily (EcoliWiki Page)	SUPERFAMILY:74650
Superfamily (EcoliWiki Page)	SUPERFAMILY:88688
Superfamily (EcoliWiki Page)	SUPERFAMILY:88713
Pfam (EcoliWiki Page)	PF07748 Glycosyl hydrolases family 38 C-terminal domain
Pfam (EcoliWiki Page)	PF09261 Alpha mannosidase, middle domain
EcoCyc	EcoCyc:EG13236	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13236	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002433	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3025	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002495	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mngB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mngB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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