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Standard Name	mlaF
Gene Synonym(s)	ECK3184, b3195, JW3162, vpsA(S.f.), yrbF^[1]^[2], vpsA, yrbF
Product Desc.	MlaF^[2]^[3]; Component of MlaF/YrbE ABC transporter^[2]^[3] Putative ATP-binding protein, ABC transporter; function unknown; mutation in the Shigella homolog is sensitive to SDS and affects virulence protein secretion^[4]
Product Synonyms(s)	predicted toluene transporter subunit: ATP-binding component of ABC superfamily^[1], B3195^[2]^[1], MlaF^[2]^[1] , ECK3184, JW3162, vpsA, yrbF, b3195
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yrbB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mlaF
Mnemonic
Synonyms	ECK3184, b3195, JW3162, vpsA(S.f.), yrbF^[1]^[2], vpsA, yrbF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	71.93 minutes	MG1655: 3338087..3337278 <gbrowseImage> name=NC_000913:3337278..3338087 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3275300..3274491 <gbrowseImage> name=NC_012967:3274491..3275300 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3224426..3225235 <gbrowseImage> name=NC_012759:3224426..3225235 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3339920..3339111 <gbrowseImage> name=NC_007779:3339111..3339920 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3435832..3435023 <gbrowseImage> name=NC_010473:3435023..3435832 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔyrbF (Keio:JW3162)	deletion	deletion			PMID:16738554	Shigen CGSC10400^[5]
ΔyrbF725::kan					PMID:16738554	CGSC:106050
mlaF(del)			Growth Phenotype	Increase outer membrane permeability.	PMID:19383799	Parental Strain: JCM158 a deriv of keo collection. Experimental Strain: JCM531	Figure 1 and 3.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3162	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAGCAGTCTGTGGCGAATTT Primer 2:CCACTCCCTGGTAAAAGATCAGC
7E3	Kohara Phage	Genobase	PMID:3038334
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[5]	est. P1 cotransduction: 82% ^[6] Synonyms:zgj-203::Tn10, zha-203::Tn10
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[5]	est. P1 cotransduction: 36% ^[6] Synonyms:zha-6::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3195
ECOL199310CYC (EcoliWiki Page)	ECOL199310CYC:C3955
ECOO157CYC (EcoliWiki Page)	ECOO157CYC:YRBF
EcoCyc (EcoliWiki Page)	ECOCYC:EG12801	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947729	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MlaF
Synonyms	predicted toluene transporter subunit: ATP-binding component of ABC superfamily^[1], B3195^[2]^[1], MlaF^[2]^[1] , ECK3184, JW3162, vpsA, yrbF, b3195
Product description	MlaF^[2]^[3]; Component of MlaF/YrbE ABC transporter^[2]^[3] Putative ATP-binding protein, ABC transporter; function unknown; mutation in the Shigella homolog is sensitive to SDS and affects virulence protein secretion^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003593	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017871	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016887	ATPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003439	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016887	ATPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017871	F	Seeded from EcoCyc (v14.0)	complete
	GO:0017111	nucleoside-triphosphatase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003593	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of YrbF/YrbE ABC transporter	could be indirect
Protein	dnaK		PMID:15690043	Experiment(s):EBI-884363
Protein	groL		PMID:15690043	Experiment(s):EBI-884363, EBI-887041
Protein	tufA		PMID:15690043	Experiment(s):EBI-884363, EBI-887041
Protein	groL		PMID:16606699	Experiment(s):EBI-1145127
Protein	hybC		PMID:15690043	Experiment(s):EBI-887041
Protein	rplL		PMID:15690043	Experiment(s):EBI-887041
Protein	tufB		PMID:19402753	MALDI(Z-score):18.307607
Protein	hybC		PMID:19402753	LCMS(ID Probability):99.4
Protein	groL		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):17.053160
Protein	groS		PMID:19402753	LCMS(ID Probability):99.6 MALDI(Z-score):27.519927
Protein	rplL		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEQSVANLVD MRDVSFTRGN RCIFDNISLT VPRGKITAIM GPSGIGKTTL LRLIGGQIAP DHGEILFDGE NIPAMSRSRL YTVRKRMSML FQSGALFTDM NVFDNVAYPL REHTQLPAPL LHSTVMMKLE AVGLRGAAKL MPSELSGGMA RRAALARAIA LEPDLIMFDE PFVGQDPITM GVLVKLISEL NSALGVTCVV VSHDVPEVLS IADHAWILAD KKIVAHGSAQ ALQANPDPRV RQFLDGIADG PVPFRYPAGD YHADLLPGS
Length	269
Mol. Wt	29.097 kDa
pI	6.6 (calculated)
Extinction coefficient	11,460 - 11,710 (calc based on 4 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	48..173	PF00005 ABC transporter		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yrbF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131085	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947729	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P63386	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947729	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1079	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	277	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	903	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yrbB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3338067..3338107 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3195 (EcoliWiki Page)	NCBI GEO profiles for yrbF
microarray	GenExpDB:b3195 (EcoliWiki Page)	Summary of data for yrbF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3337633..3337999) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ14; Well:G12^[7]
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Notes

Accessions Related to mlaF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3195
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT1G65410 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0031362 (score: 1.000; bootstrap: 69%) FBgn0032167 (score: 0.229) FBgn0025683 (score: 0.225) FBgn0020762 (score: 0.211) FBgn0031516 (score: 0.208) FBgn0031220 (score: 0.184) FBgn0052091 (score: 0.175) FBgn0031449 (score: 0.159) FBgn0031515 (score: 0.153) FBgn0003996 (score: 0.077) FBgn0003515 (score: 0.074) FBgn0020445 (score: 0.059)	From Inparanoid:20070104
Drosophila pseudoobscura	GA14594-PA (score: 1.000; bootstrap: 57%) GA16356-PA (score: 0.236) GA15545-PA (score: 0.235) GA19180-PA (score: 0.225) GA18458-PA (score: 0.185) GA16671-PA (score: 0.176) GA16397-PA (score: 0.154) GA15454-PA (score: 0.057)	From Inparanoid:20070104
Oryza gramene	Q6ASX4 (score: 1.000; bootstrap: 97%) Q7Y189 (score: 0.844)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000013943 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	VPSA	From SHIGELLACYC
E. coli O157	YRBF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00005 ABC transporter
Panther (EcoliWiki Page)	PTHR24220:SF52
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

mlaF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mlaF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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