mhpD:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mhpD

Mnemonic

m-Hydroxyphenylpropionic acid

Synonyms

ECK0347, b0350, JW0341, mhpS, G6204[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

8 minutes 

MG1655: 371339..372148
<gbrowseImage> name=NC_000913:371339..372148 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 344671..345480
<gbrowseImage> name=NC_012967:344671..345480 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 274524..274907
<gbrowseImage> name=NC_012759:274524..274907 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 371339..372148
<gbrowseImage> name=NC_007779:371339..372148 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

371342

Edman degradation

PMID:9492273[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mhpD(del) (Keio:JW0341)

deletion

deletion

PMID:16738554[4]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0341

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACGAAGCATACTCTTGAGCA

Primer 2:CCTGACAGACTTCCTTTTGGGGC

mhpC281::Tn10

Linked marker

CAG12080 = CGSC7333[6]

est. P1 cotransduction: 99% [7]
Synonyms:zah-281::Tn10

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[6]

est. P1 cotransduction: 42% [7]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:M013

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14274

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004472

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944768

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4022

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001205

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Pollard, JR & Bugg, TD (1998) Purification, characterisation and reaction mechanism of monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli. Eur. J. Biochem. 251 98-106 PubMed
  4. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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