mglB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mglB

Mnemonic

Methyl-galactoside

Synonyms

ECK2143, b2150, JW2137[1], JW2137

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.22 minutes, 48.22 minutes 

MG1655: 2238370..2237372
<gbrowseImage> name=NC_000913:2237372..2238370 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2191586..2190588
<gbrowseImage> name=NC_012967:2190588..2191586 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2129855..2130853
<gbrowseImage> name=NC_012759:2129855..2130853 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2243682..2242684
<gbrowseImage> name=NC_007779:2242684..2243682 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2329359..2328361
<gbrowseImage> name=NC_010473:2328361..2329359 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2237375

Edman degradation

PMID:7012152[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mglBG97D

G97D

Improductive interaction with trg

seeded from UniProt:P0AEE5

ΔmglB (Keio:JW2137)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9732[5]

mglB::Tn5KAN-2 (FB20653)

Insertion at nt 322 in Minus orientation

PMID:15262929[6]

E. coli Genome Project:FB20653

contains pKD46

mglB::Tn5KAN-2 (FB20654)

Insertion at nt 322 in Minus orientation

PMID:15262929[6]

E. coli Genome Project:FB20654

does not contain pKD46

mglB550

CGSC:73076

ΔmglB777::kan

PMID:16738554[4]

CGSC:101850


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2137

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAATAAGAAGGTGTTAACCCT

Primer 2:CCTTTCTTGCTGAATTCAGCCAG

7F1

Kohara Phage

Genobase

PMID:3038334[8]

7H12

Kohara Phage

Genobase

PMID:3038334[8]

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[5]

est. P1 cotransduction: 4% [9]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[5]

est. P1 cotransduction: 3% [9]
Synonyms:zeg-722, zei-722::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10593

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10593

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000586

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:949041

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0588

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007108

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Mahoney, WC et al. (1981) The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r. J. Biol. Chem. 256 4350-6 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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