mdtI:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
mdtI |
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Gene Synonym(s) |
ECK1594, b1599, JW1591, mtdI, ydgE[1], ydgE |
Product Desc. |
Component of MdtIJ transporter complex[2][3] Multidrug resistance efflux protein, with MdtJ; overexpression resistance to deoxycholate, SDS, nalidixic acid and fosfomycin[4] |
Product Synonyms(s) |
multidrug efflux system transporter[1], MdtI[2][1], B1599[2][1], YdgE[2][1] , ECK1594, JW1591, ydgE, b1599 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
MdtI belongs to the SMR efflux pump family, DMT superfamily. MdtI has been topology mapped using protein fusions. MdtJI exports spermidine (HIgashi, 2007).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
mdtI |
---|---|
Mnemonic |
Multidrug transporter |
Synonyms |
ECK1594, b1599, JW1591, mtdI, ydgE[1], ydgE |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
36.01 minutes |
MG1655: 1671173..1670844 |
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NC_012967: 1650015..1650344 |
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NC_012759: 1562903..1563232 |
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W3110 |
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W3110: 1674863..1674534 |
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DH10B: 1762134..1761805 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔmdtI (Keio:JW1591) |
deletion |
deletion |
PMID:16738554 |
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mdtIE5Q |
E5Q |
Decrease in spermidine excretion |
seeded from UniProt:P69210 | ||||
mdtIE19Q |
E19Q |
Decrease in spermidine excretion |
seeded from UniProt:P69210 | ||||
mdtIW68L |
W68L |
Decrease in spermidine excretion |
seeded from UniProt:P69210 | ||||
mdtIW81L |
W81L |
Decrease in spermidine excretion |
seeded from UniProt:P69210 | ||||
mdtID60N |
D60N |
Decrease in spermidine excretion |
seeded from UniProt:P69210 | ||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW1591 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCGCGCAGTTTGAATGGGTTCA Primer 2:CCGGCAAGTTTCACCATGATCAT | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: % [6] | ||
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: % [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:G6857 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG13926 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120003446 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB3685 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0005343 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
MdtI |
---|---|
Synonyms |
multidrug efflux system transporter[1], MdtI[2][1], B1599[2][1], YdgE[2][1] , ECK1594, JW1591, ydgE, b1599 |
Product description |
Component of MdtIJ transporter complex[2][3] Multidrug resistance efflux protein, with MdtJ; overexpression resistance to deoxycholate, SDS, nalidixic acid and fosfomycin[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005887 |
integral to plasma membrane |
PMID:11867724 |
IDA: Inferred from Direct Assay |
C |
complete | |||
GO:0015606 |
spermidine transmembrane transporter activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01597 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0015848 |
spermidine transport |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_01597 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of MdtIJ transporter complex |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
plasma membrane |
C-terminus localized to the periplasm with 4 predicted transmembrane domains |
Daley et al. (2005) [7] |
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plasma membrane |
From EcoCyc[3] |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MAQFEWVHAA WLALAIVLEI VANVFLKFSD GFRRKIFGLL SLAAVLAAFS ALSQAVKGID LSVAYALWGG FGIAATLAAG WILFGQRLNR KGWIGLVLLL AGMIMVKLA |
Length |
109 |
Mol. Wt |
11.72 kDa |
pI |
10.7 (calculated) |
Extinction coefficient |
28,990 (calc based on 1 Y, 5 W, and 0 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0005343 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:G6857 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG13926 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120003446 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB3685 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MG1655 |
104 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
26 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
108a |
molecules/cell/generation |
|
Ribosome Profiling |
Low confidence in the sequencing data set. |
PMID: 24766808 |
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:1671153..1671193
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for mdtI | |
microarray |
Summary of data for mdtI from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to mdtI Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:G6857 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB3685 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG13926 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120003446 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0005343 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
S1752 |
From SHIGELLACYC |
E. coli O157 |
Z2593 |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:G6857 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG13926 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120003446 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB3685 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0005343 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
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