malK:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
malK |
|---|---|
| Mnemonic |
Maltose |
| Synonyms |
ECK4027, b4035, JW3995, malB[1] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
91.49 minutes |
MG1655: 4244807..4245922 |
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NC_012967: 4225716..4226831 |
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NC_012759: 4183652..4184767 |
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W3110 |
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W3110: 4250374..4251489 |
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DH10B: 4344503..4345618 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmalK (Keio:JW3995) |
deletion |
deletion |
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malKG137A |
G137A |
Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type malK |
seeded from UniProt:P68187 | ||||
|
malKA124T |
A124T |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKA85M |
A85M |
Suppressor of EAA loop mutations in MalFG |
seeded from UniProt:P68187 | ||||
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malKK106C |
K106C |
Suppressor of EAA loop mutations in MalFG |
seeded from UniProt:P68187 | ||||
|
malKV114C |
V114C |
Suppressor of EAA loop mutations in MalFG |
seeded from UniProt:P68187 | ||||
|
malKR228C |
R228C |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKF241I |
F241I |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKW267G |
W267G |
Normal maltose transport but constitutive mal gene expression |
seeded from UniProt:P68187 | ||||
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malKD158N |
D158N |
Loss of maltose transport but retains ability to repress mal genes |
seeded from UniProt:P68187 | ||||
|
malKG340A |
G340A |
Maltose transport is affected but retains ability to interact with malT |
seeded from UniProt:P68187 | ||||
|
malKG278P |
G278P |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKS282L |
S282L |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKG284S |
G284S |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
|
malKG302D |
G302D |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
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malKE308Q |
E308Q |
Maltose transport is affected but retains ability to interact with malT |
seeded from UniProt:P68187 | ||||
|
malKS322F |
S322F |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
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malKG346S |
G346S |
Normal maltose transport but constitutive mal gene expression |
seeded from UniProt:P68187 | ||||
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malKF355Y |
F355Y |
Maltose transport is affected but retains ability to interact with malT |
seeded from UniProt:P68187 | ||||
|
malKV117M |
V117M |
Suppressor of EAA loop mutations in MalFG |
seeded from UniProt:P68187 | ||||
|
malKE119K |
E119K |
Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity |
seeded from UniProt:P68187 | ||||
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malK62(Am) |
amber (UAG) mutation | ||||||
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malK58::Tn5 |
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malK27 |
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ΔmalK731::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3995 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCGAGCGTACAGCTGCAAAA Primer 2:CCAACGCCCGGCTCCTTATGCAG | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 87% [6] | ||
|
Linked marker |
est. P1 cotransduction: 4% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
