malG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malG

Mnemonic

Maltose

Synonyms

ECK4024, b4032, JW3992[1], JW3992, malJ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.4 minutes 

MG1655: 4241539..4240649
<gbrowseImage> name=NC_000913:4240649..4241539 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4222448..4221558
<gbrowseImage> name=NC_012967:4221558..4222448 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4130329..4131300
<gbrowseImage> name=NC_012759:4130329..4131300 source=BW2952 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4180361..4180384
<gbrowseImage> name=NC_012759:4180361..4180384 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4247106..4246216
<gbrowseImage> name=NC_007779:4246216..4247106 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4341235..4340345
<gbrowseImage> name=NC_010473:4340345..4341235 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

malG(del) (Keio:JW3992)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC10873[3]

malGA192D,S,L

A192D,S,L

Loss of transport and malK dissociation from the membrane

seeded from UniProt:P68183

malGG196A

G196A

No effect

seeded from UniProt:P68183

malGG196P

G196P

Loss of transport and malK dissociation from the membrane

seeded from UniProt:P68183

malGP209A

P209A

No effect

seeded from UniProt:P68183

malGE190A,C,K,L

E190A,C,K,L

Reduction of transport rate

seeded from UniProt:P68183

malG53::Tn10

CGSC:7073

malG67

CGSC:9965

malG728(del)::kan

PMID:16738554[2]

CGSC:101734

malG(del)

deletion

deletion

Resistant to

Thio(5)maltose increase resistance.

PMID:6998971[4]

Selection for mutant maltose transport system.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3992

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCAATGGTCCAACCGAAATC

Primer 2:CCACCTTTCACACCACCTGCCGT

7A4

Kohara Phage

Genobase

PMID:3038334[6]

1F8

Kohara Phage

Genobase

PMID:3038334[6]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 13% [7]
Synonyms:thi-39::Tn10

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[3]

est. P1 cotransduction: 100% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10556

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10556

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000549

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948530

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0551

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013193

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Ferenci, T (1980) Methyl-alpha-maltoside and 5-thiomaltose: analogs transported by the Escherichia coli maltose transport system. J. Bacteriol. 144 7-11 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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