lysP:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lysP

Mnemonic

Lysine

Synonyms

ECK2149, b2156, JW2143, cadR[1], cadR

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

48.39 minutes 

MG1655: 2246554..2245085
<gbrowseImage> name=NC_000913:2245085..2246554 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2199770..2198301
<gbrowseImage> name=NC_012967:2198301..2199770 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2137568..2139037
<gbrowseImage> name=NC_012759:2137568..2139037 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2251866..2250397
<gbrowseImage> name=NC_007779:2250397..2251866 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2337543..2336074
<gbrowseImage> name=NC_010473:2336074..2337543 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2245088

Edman degradation

PMID:7551055[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔlysP (Keio:JW2143)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9737[4]

lysP::Tn5KAN-2 (FB20657)

Insertion at nt 946 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20657

contains pKD46

lysP::Tn5KAN-2 (FB20658)

Insertion at nt 946 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20658

does not contain pKD46

lysP81

CGSC:9587

lysP37

PMID:6767681[6]

CGSC:10673

ΔlysP783::kan

PMID:16738554[3]

CGSC:101710


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2143

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGTTTCCGAAACTAAAACCAC

Primer 2:CCTTTCTTATCGTTCTGCGGGAA

7F1

Kohara Phage

Genobase

PMID:3038334[8]

7H12

Kohara Phage

Genobase

PMID:3038334[8]

5H11

Kohara Phage

Genobase

PMID:3038334[8]

4F2

Kohara Phage

Genobase

PMID:3038334[8]

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[4]

est. P1 cotransduction: 2% [9]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[4]

est. P1 cotransduction: 6% [9]
Synonyms:zeg-722, zei-722::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11337

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11337

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001308

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946667

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1313

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007131

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Ellis, J et al. (1995) Topological analysis of the lysine-specific permease of Escherichia coli. Microbiology (Reading, Engl.) 141 ( Pt 8) 1927-35 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Popkin, PS & Maas, WK (1980) Escherichia coli regulatory mutation affecting lysine transport and lysine decarboxylase. J. Bacteriol. 141 485-92 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 8.2 8.3 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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