lpxA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lpxA

Mnemonic

Lipid A expression/biosynthesis

Synonyms

ECK0180, b0181, JW0176[1], JW0176

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.37 minutes 

MG1655: 202560..203348
<gbrowseImage> name=NC_000913:202560..203348 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 205402..206190
<gbrowseImage> name=NC_012967:205402..206190 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 202559..203347
<gbrowseImage> name=NC_012759:202559..203347 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 202560..203348
<gbrowseImage> name=NC_007779:202560..203348 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 176664..177452
<gbrowseImage> name=NC_010473:176664..177452 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

202560

Edman degradation

PMID:8366124[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lpxAG189S

G189S

(in strain: SM101; temperature- sensitive)

Strain variation; seeded from UniProt:P0A722

lpxA2(ts)

temperature sensitive

CGSC:26548


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0176

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCATTGATAAATCCGCCTTTGT

Primer 2:CCACGAATCAGACCGCGCGTTGA

E2B8

Kohara Phage

Genobase

PMID:3038334[4]

6F5

Kohara Phage

Genobase

PMID:3038334[4]

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 7% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 32% [6]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10545

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10545

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000538

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944849

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0540

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000618

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Anderson, MS et al. (1993) UDP-N-acetylglucosamine acyltransferase of Escherichia coli. The first step of endotoxin biosynthesis is thermodynamically unfavorable. J. Biol. Chem. 268 19858-65 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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