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Standard Name	ligB
Gene Synonym(s)	ECK3637, b3647, JW3622, yicF^[1], yicF
Product Desc.	DNA ligase^[2]^[3] DNA ligase B, NAD(+)-dependent^[4]
Product Synonyms(s)	DNA ligase, NAD(+)-dependent^[1], B3647^[2]^[1], LigB^[2]^[1], YicF^[2]^[1] , ECK3637, JW3622, yicF, b3647
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ligB^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ligB
Mnemonic	Ligase B
Synonyms	ECK3637, b3647, JW3622, yicF^[1], yicF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	82.28 minutes, 82.28 minutes	MG1655: 3819193..3817511 <gbrowseImage> name=NC_000913:3817511..3819193 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3757845..3759527 <gbrowseImage> name=NC_012967:3757845..3759527 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3705838..3707520 <gbrowseImage> name=NC_012759:3705838..3707520 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3819245..3820927 <gbrowseImage> name=NC_007779:3819245..3820927 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3916771..3915089 <gbrowseImage> name=NC_010473:3915089..3916771 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔligB (Keio:JW3622)	deletion	deletion		PMID:16738554	Shigen CGSC10663^[5]
ligBK124A		K124A	Loss of activity			seeded from UniProt:P25772
ΔligB753::kan				PMID:16738554	CGSC:101660
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3622	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGTATGGATGGCGATATT Primer 2:CCAGGTTCAAAACCTGTGATCTG
7F3	Kohara Phage	Genobase	PMID:3038334
2A6	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 70% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11334	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001305	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948164	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1310	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011923	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LigB
Synonyms	DNA ligase, NAD(+)-dependent^[1], B3647^[2]^[1], LigB^[2]^[1], YicF^[2]^[1] , ECK3637, JW3622, yicF, b3647
Product description	DNA ligase^[2]^[3] DNA ligase B, NAD(+)-dependent^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	PMID:11812821	IDA: Inferred from Direct Assay		F		complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01587	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004150	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013839	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013840	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018239	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020923	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003911	DNA ligase (NAD+) activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.5.1.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004150	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018239	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004150	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018239	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006281	DNA repair	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0234	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0227	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0464	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMMKVWMAIL IGILCWQSSV WAVCPAWSPA RAQEEISRLQ QQIKQWDDDY WKEGKSEVED GVYDQLSARL TQWQRCFGSE PRDVMMPPLN GAVMHPVAHT GVRKMVDKNA LSLWMRERSD LWVQPKVDGV AVTLVYRDGK LNKAISRGNG LKGEDWTQKV SLISAVPQTV SGPLANSTLQ GEIFLQREGH IQQQMGGINA RAKVAGLMMR QDDSDTLNSL GVFVWAWPDG PQLMSDRLKE LATAGFTLTQ TYTRAVKNAD EVARVRNEWW KAELPFVTDG VVVRAAKEPE SRHWLPGQAE WLVAWKYQPV AQVAEVKAIQ FAVGKSGKIS VVASLAPVML DDKKVQRVNI GSVRRWQEWD IAPGDQILVS LAGQGIPRID DVVWRGAERT KPTPPENRFN SLTCYFASDV CQEQFISRLV WLGAKQVLGL DGIGEAGWRA LHQTHRFEHI FSWLLLTPEQ LQNTPGIAKS KSAQLWHQFN LARKQPFTRW VMAMGIPLTR AALNASDERS WSQLLFSTEQ FWQQLPGTGS GRARQVIEWK ENAQIKKLGS WLAAQQITGF EP
Length	562
Mol. Wt	63.44 kDa
pI	9.3 (calculated)
Extinction coefficient	168,440 - 169,065 (calc based on 6 Y, 29 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	28..308	PF01653 NAD-dependent DNA ligase adenylation domain	PMID:19920124
Domain	310..393	PF03120 NAD-dependent DNA ligase OB-fold domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ligB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111628	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948164	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011923	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P25772	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11334	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948164	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001305	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1310	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ligB
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3819173..3819213 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3647 (EcoliWiki Page)	NCBI GEO profiles for ligB
microarray	GenExpDB:b3647 (EcoliWiki Page)	Summary of data for ligB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3818734..3819098) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:E3^[7]
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Notes

Accessions Related to ligB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11334	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1310	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3647
EcoGene	EcoGene:EG11334	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001305	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011923	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YICF	From SHIGELLACYC
E. coli O157	YICF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03120 NAD-dependent DNA ligase OB-fold domain
Pfam (EcoliWiki Page)	PF01653 NAD-dependent DNA ligase adenylation domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:47781
Superfamily (EcoliWiki Page)	SUPERFAMILY:50249
Panther (EcoliWiki Page)	PTHR11107:SF2
Superfamily (EcoliWiki Page)	SUPERFAMILY:56091
EcoCyc	EcoCyc:EG11334	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11334	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001305	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1310	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011923	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

ligB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ligB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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