leuW:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

leuW

Gene Synonym(s)

ECK0660, b0672, JWR0016, feeB[1], feeB

Product Desc.


Product Synonyms(s)

tRNA-Leu(UAG) (Leucine tRNA3)[1]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): metT-leuW-glnUW-metU-glnVX[2], metT

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

leuW

Mnemonic

Leucine

Synonyms

ECK0660, b0672, JWR0016, feeB[1], feeB

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

15.01 minutes 

MG1655: 696270..696186
<gbrowseImage> name=NC_000913:696186..696270 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 679058..679142
<gbrowseImage> name=NC_012967:679058..679142 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 598946..599030
<gbrowseImage> name=NC_012759:598946..599030 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 697469..697385
<gbrowseImage> name=NC_007779:697385..697469 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 748862..748778
<gbrowseImage> name=NC_010473:748778..748862 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

16A8

Kohara Phage

Genobase

PMID:3038334

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[3]

est. P1 cotransduction: 16% [4]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[3]

est. P1 cotransduction: 86% [4]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30052

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002494

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945264

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4215

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

tRNA-Leu(UAG) (Leucine tRNA3)

Synonyms

tRNA-Leu(UAG) (Leucine tRNA3)[1]

Product description


EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties

Type

tRNA

Sequence
GCGGGAGUGG CGAAAUUGGU AGACGCACCA GAUUUAGGUU CUGGCGCCGC AAGGUGUGCG
AGUUCAAGUC UCGCCUCCCG CACCA
Mol. Wt

27645.48


Domains/Motifs/Modification Sites See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map />

Image couresy of Rfam


Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=leuW taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30052

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002494

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4215

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

metT-leuW-glnUW-metU-glnVX

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:696250..696290 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0672 (EcoliWiki Page)

NCBI GEO profiles for leuW

microarray

GenExpDB:b0672 (EcoliWiki Page)

Summary of data for leuW from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to leuW Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30052

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4215

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0672

EcoGene

EcoGene:EG30052

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002494

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002288

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

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Do-It-Yourself Web Tools

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Notes

Families

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Database Accession Notes

PFAM (EcoliWiki Page)

PFAM:PF01360

edit table

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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