PMID:7028742
| Citation |
Newman, MJ, Foster, DL, Wilson, TH and Kaback, HR (1981) Purification and reconstitution of functional lactose carrier from Escherichia coli. J. Biol. Chem. 256:11804-8 |
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| Abstract |
The lactose carrier protein of Escherichia coli was purified by a simple procedure employing differential solubilization and ion-exchange chromatography and reconstituted into liposomes by octylglucoside dilution. The proteoliposomes exhibited both membrane potential-driven lactose transport and lactose counterflow. Furthermore, the purified protein was identified as the product of the lac y gene. These and other results demonstrate that the lactose carrier is the only polypeptide species essential for energy-coupled lactose transport and counterflow. |
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| Keywords |
Amino Acids/analysis; Cell Membrane/enzymology; Escherichia coli/enzymology; Escherichia coli Proteins; Kinetics; Membrane Potentials; Membrane Transport Proteins/isolation & purification; Membrane Transport Proteins/metabolism; Molecular Weight; Monosaccharide Transport Proteins; Symporters |
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