PMID:7000781

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Citation

Newman, MJ and Wilson, TH (1980) Solubilization and reconstitution of the lactose transport system from Escherichia coli. J. Biol. Chem. 255:10583-6

Abstract

The lactose transport system from Escherichia coli was solubilized with octylglucoside and reconstituted into liposomes by an octylglucoside dilution procedure. The reconstituted proteoliposomes exhibited lactose counterflow and membrane potential-driven lactose transport.

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Keywords

Escherichia coli/metabolism; Escherichia coli Proteins; Kinetics; Lactose/metabolism; Liposomes; Membrane Lipids/isolation & purification; Membrane Lipids/metabolism; Membrane Transport Proteins/metabolism; Monosaccharide Transport Proteins; Solubility; Symporters

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