PMID:7000781
Citation |
Newman, MJ and Wilson, TH (1980) Solubilization and reconstitution of the lactose transport system from Escherichia coli. J. Biol. Chem. 255:10583-6 |
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Abstract |
The lactose transport system from Escherichia coli was solubilized with octylglucoside and reconstituted into liposomes by an octylglucoside dilution procedure. The reconstituted proteoliposomes exhibited lactose counterflow and membrane potential-driven lactose transport. |
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Keywords |
Escherichia coli/metabolism; Escherichia coli Proteins; Kinetics; Lactose/metabolism; Liposomes; Membrane Lipids/isolation & purification; Membrane Lipids/metabolism; Membrane Transport Proteins/metabolism; Monosaccharide Transport Proteins; Solubility; Symporters |
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