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Standard Name	kefF
Gene Synonym(s)	ECK0047, b0046, JW0045, yabF^[1], yabF
Product Desc.	regulator of KefC-mediated potassium transport^[2]^[3] Required for KefC-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for activity^[4]
Product Synonyms(s)	flavoprotein subunit for the KefC potassium efflux system^[1], B0046^[2]^[1], KefF^[2]^[1], YabF^[2]^[1] , ECK0047, JW0045, yabF, b0046
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): kefF^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	kefF
Mnemonic	K+ efflux
Synonyms	ECK0047, b0046, JW0045, yabF^[1], yabF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.02 minutes	MG1655: 47246..47776 <gbrowseImage> name=NC_000913:47246..47776 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 51441..51971 <gbrowseImage> name=NC_012967:51441..51971 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 47246..47776 <gbrowseImage> name=NC_012759:47246..47776 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 47246..47776 <gbrowseImage> name=NC_007779:47246..47776 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 47246..47776 <gbrowseImage> name=NC_010473:47246..47776 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

kefF(del) (Keio:JW0045)

deletion

PMID:16738554

Shigen

CGSC8358^[5]

kefF759(del)::kan

PMID:16738554

CGSC:101628

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0045	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTCTTATAATTTATGCGCA Primer 2:CCTCCATGATGGGCCTCCTGCCA
2F7	Kohara Phage	Genobase	PMID:3038334
4A3	Kohara Phage	Genobase	PMID:3038334
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 59% ^[6] Synonyms:car-96::Tn10
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 23% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11568	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11568	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944767	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001520	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1528	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000159	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	KefF
Synonyms	flavoprotein subunit for the KefC potassium efflux system^[1], B0046^[2]^[1], KefF^[2]^[1], YabF^[2]^[1] , ECK0047, JW0045, yabF, b0046
Product description	regulator of KefC-mediated potassium transport^[2]^[3] Required for KefC-mediated K+ efflux; member of quinone oxidoreductase family, but lacks key residues needed for activity^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006813	potassium ion transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01414	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050662	coenzyme binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003680	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009055	electron carrier activity	PMID:17679694	IMP: Inferred from Mutant Phenotype		F	Fig 4	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	fhlA	PMID:16606699	Experiment(s):EBI-1135438
Protein	sdhA	PMID:16606699	Experiment(s):EBI-1135438
Protein	thrS	PMID:16606699	Experiment(s):EBI-1135438
Protein	groL	PMID:16606699	Experiment(s):EBI-1135438
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MILIIYAHPY PHHSHANKRM LEQARTLEGV EIRSLYQLYP DFNIDIAAEQ EALSRADLIV WQHPMQWYSI PPLLKLWIDK VFSHGWAYGH GGTALHGKHL LWAVTTGGGE SHFEIGAHPG FDVLSQPLQA TAIYCGLNWL PPFAMHCTFI CDDETLEGQA RHYKQRLLEW QEAHHG
Length	176
Mol. Wt	20.169 kDa
pI	6.3 (calculated)
Extinction coefficient	50,420 - 50,795 (calc based on 8 Y, 7 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..167	PF02525 Flavodoxin-like fold		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kefF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128040	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000159	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A754	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11568	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11568	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944767	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001520	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944767	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1528	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	101	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	91	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	60^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
kefF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:47226..47266 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0046 (EcoliWiki Page)	NCBI GEO profiles for kefF
microarray	GenExpDB:b0046 (EcoliWiki Page)	Summary of data for kefF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (47050..47270) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:A3^[7]
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Notes

Accessions Related to kefF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11568	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1528	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0046
EcoGene	EcoGene:EG11568	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001520	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000159	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000402 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000034446 (score: 1.000; bootstrap: 100%) ENSAPMP00000022018 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000039940 (score: 1.000; bootstrap: 100%) ENSBTAP00000032251 (score: 0.410)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000013713 (score: 1.000; bootstrap: 100%) ENSCAFP00000030044 (score: 0.362)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030131-1226 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-1145 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000020892 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000337773 (score: 1.000; bootstrap: 100%) ENSP00000319788 (score: 0.359)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000011126 (score: 1.000; bootstrap: 100%) ENSMMUP00000025865 (score: 0.360)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000003283 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:103187 (score: 1.000; bootstrap: 100%) MGI:104513 (score: 0.320)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000030178 (score: 1.000; bootstrap: 100%) ENSPTRP00000014148 (score: 0.361)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000017175 (score: 1.000; bootstrap: 100%) ENSRNOP00000024141 (score: 0.338) ENSRNOP00000033201 (score: 0.303)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000145774 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YABF	From SHIGELLACYC
E. coli O157	YABF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02525 Flavodoxin-like fold
Superfamily (EcoliWiki Page)	SUPERFAMILY:52218
Panther (EcoliWiki Page)	PTHR10204:SF0
EcoCyc	EcoCyc:EG11568	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11568	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001520	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1528	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000159	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

kefF:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to kefF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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