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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	kch
Gene Synonym(s)	ECK1244, b1250, JW1242^[1], JW1242
Product Desc.	potassium VIC channel^[2]^[3] Voltage-gated potassium channel protein; overproduction increases the permeability of the cytoplamsic membrane to K+; not involved in K+ uptake; overproduction alters membrane composition; homotetrameric membrane protein^[4]
Product Synonyms(s)	voltage-gated potassium channel^[1], B1250^[2]^[1], Kch^[2]^[1] , ECK1244, JW1242, b1250
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): kch^[2]
Regulation/Activity
Quick Links
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Notes

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Consistently co-purifies with the degradosome.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	kch
Mnemonic	Potassium (K+) channel
Synonyms	ECK1244, b1250, JW1242^[1], JW1242
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	28.17 minutes	MG1655: 1308293..1307040 <gbrowseImage> name=NC_000913:1307040..1308293 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1307863..1306610 <gbrowseImage> name=NC_012967:1306610..1307863 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1197889..1199142 <gbrowseImage> name=NC_012759:1197889..1199142 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1311983..1310730 <gbrowseImage> name=NC_007779:1310730..1311983 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1349302..1348115 <gbrowseImage> name=NC_010473:1348115..1349302 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 1348870..1348649 <gbrowseImage> name=NC_010473:1348649..1348870 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 1348576..1348217 <gbrowseImage> name=NC_010473:1348217..1348576 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 1397689..1396986 <gbrowseImage> name=NC_010473:1396986..1397689 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 1349311..1348058 <gbrowseImage> name=NC_010473:1348058..1349311 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
Δkch (Keio:JW1242)	deletion	deletion		PMID:16738554	Shigen CGSC9121^[5]
kchS411N		S411N	(in strain: ECOR 46)			Strain variation; seeded from UniProt:P31069
kchA415V		A415V	(in strain: ECOR 28)			Strain variation; seeded from UniProt:P31069
kchA5T		A5T	(in strain: ECOR 52 and ECOR 60)			Strain variation; seeded from UniProt:P31069
kchN13K		N13K	(in strain: ECOR 52 and ECOR 60)			Strain variation; seeded from UniProt:P31069
kchP223S		P223S	(in strain: ECOR 4)			Strain variation; seeded from UniProt:P31069
kchL405Q		L405Q	(in strain: ECOR 4, ECOR 16, ECOR 28, ECOR 31, ECOR 37, ECOR 46, ECOR 50, ECOR 52, ECOR 60 and ECOR 71)			Strain variation; seeded from UniProt:P31069
kchE406K		E406K	(in strain: ECOR 46)			Strain variation; seeded from UniProt:P31069
kchS414L		S414L	(in strain: ECOR 46)			Strain variation; seeded from UniProt:P31069
Δkch-758::kan				PMID:16738554	CGSC:101600
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1242	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTCACTGGGCTACATTCAA Primer 2:CCTTTTTGCGCCGATTCTTTACT
14C4	Kohara Phage	Genobase	PMID:3038334
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 90% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
trpC83::Tn10	Linked marker	CAG18455 = CGSC7371^[5]	est. P1 cotransduction: 69% ^[6] Synonyms:trpB83::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11606	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11606	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001554	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945841	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1563	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Kch
Synonyms	voltage-gated potassium channel^[1], B1250^[2]^[1], Kch^[2]^[1] , ECK1244, JW1242, b1250
Product description	potassium VIC channel^[2]^[3] Voltage-gated potassium channel protein; overproduction increases the permeability of the cytoplamsic membrane to K+; not involved in K+ uptake; overproduction alters membrane composition; homotetrameric membrane protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005488	binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006813	potassium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003148	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008152	metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016040	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	iscA		PMID:19402753	MALDI(Z-score):23.648996
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 6 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSHWATFKQT ATNLWVTLRH DILALAVFLN GLLIFKTIYG MSVNLLDIFH IKAFSELDLS LLANAPLFML GVFLVLNSIG LLFRAKLAWA ISIILLLIAL IYTLHFYPWL KFSIGFCIFT LVFLLILRKD FSHSSAAAGT IFAFISFTTL LFYSTYGALY LSEGFNPRIE SLMTAFYFSI ETMSTVGYGD IVPVSESARL FTISVIISGI TVFATSMTSI FGPLIRGGFN KLVKGNNHTM HRKDHFIVCG HSILAINTIL QLNQRGQNVT VISNLPEDDI KQLEQRLGDN ADVIPGDSND SSVLKKAGID RCRAILALSD NDADNAFVVL SAKDMSSDVK TVLAVSDSKN LNKIKMVHPD IILSPQLFGS EILARVLNGE EINNDMLVSM LLNSGHGIFS DNDELETKAD SKESAQK
Length	417
Mol. Wt	46.065 kDa
pI	6.6 (calculated)
Extinction coefficient	33,920 - 34,295 (calc based on 8 Y, 4 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	246..365	PF02254 TrkA-N domain	PMID:19920124
Domain	148..226	PF07885 Ion channel	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kch taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129211	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945841	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004189	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31069	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11606	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11606	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945841	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001554	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1563	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	29.372+/-0.187	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.028731045	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	120	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	123	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	65	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
kch
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1308273..1308313 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1250 (EcoliWiki Page)	NCBI GEO profiles for kch
microarray	GenExpDB:b1250 (EcoliWiki Page)	Summary of data for kch from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1308206..1308637) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:F1^[8]
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Notes

Accessions Related to kch Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11606	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1563	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1250
EcoGene	EcoGene:EG11606	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001554	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000021317 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000017701 (score: 1.000; bootstrap: 100%) ENSBTAP00000038666 (score: 0.115)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00033787 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00004830 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000022780 (score: 1.000; bootstrap: 100%) ENSCAFP00000009342 (score: 0.554) ENSCAFP00000015336 (score: 0.091) ENSCAFP00000029217 (score: 0.085)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0003429 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10500-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000001142 (score: 1.000; bootstrap: 100%) ENSMODP00000013713 (score: 0.258)	From Inparanoid:20070104
Shigella flexneri	KCH	From SHIGELLACYC
E. coli O157	KCH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:51735
Panther (EcoliWiki Page)	PTHR11537:SF103
Superfamily (EcoliWiki Page)	SUPERFAMILY:81324
Pfam (EcoliWiki Page)	PF02254 TrkA-N domain
Pfam (EcoliWiki Page)	PF07885 Ion channel
EcoCyc	EcoCyc:EG11606	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11606	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001554	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1563	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

kch:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to kch Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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