iraM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

iraM

Mnemonic
Synonyms

ECK1147, b1160, JW1147, ycgW, elb1, elbA[1], elb1, elbA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.1 minutes 

MG1655: 1211226..1210903
<gbrowseImage> name=NC_000913:1210903..1211226 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1099624..1099947
<gbrowseImage> name=NC_012759:1099624..1099947 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1213580..1213257
<gbrowseImage> name=NC_007779:1213257..1213580 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1251467..1251144
<gbrowseImage> name=NC_010473:1251144..1251467 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔelbA (Keio:JW1147)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9066[3]

zcf-117::Tn10

Insertion at 1,211,084 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG18463 = CGSC7363[3]

Synonyms:

ΔelbA749::kan

PMID:16738554[2]

CGSC:100694


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1147

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAGTGGATAGTAATTGACAC

Primer 2:CCtTTGAGCCCATATGGGCATAT

3E11

Kohara Phage

Genobase

PMID:3038334[6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[3]

est. P1 cotransduction: 6% [4]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 42% [4]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1160

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1429

EcoCyc (EcoliWiki Page)

ECOCYC:G6600

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945729

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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