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On One Page

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Expression|

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Standard Name	intE
Gene Synonym(s)	ECK1126, b1140, JW1126, int(Lambda), ycfI^[1], int, ycfI
Product Desc.	e14 prophage; predicted integrase^[2]^[3] e14 integrase, in defective prophage^[4]
Product Synonyms(s)	e14 prophage; predicted integrase^[1], B1140^[2]^[1], YcfI^[2]^[1], IntE^[2]^[1] , ECK1126, int, JW1126, ycfI, b1140
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): intE^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	intE
Mnemonic	Integrase
Synonyms	ECK1126, b1140, JW1126, int(Lambda), ycfI^[1], int, ycfI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain

Map location

Genome coordinates

Genome browsers

Sequence links

MG1655

25.84 minutes

MG1655: 1200029..1198902
<gbrowseImage> name=NC_000913:1198902..1200029 source=MG1655 preset=GeneLocation </gbrowseImage>

EcoCyc

GBrowse(MG1655)

W3110

W3110: 1202383..1201256
<gbrowseImage> name=NC_007779:1201256..1202383 source=W3110 preset=GeneLocation </gbrowseImage>

GBrowse(W3110)

FASTA Dump

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔintE (Keio:JW1126)	deletion	deletion	PMID:16738554	Shigen CGSC9053^[5]
ΔintE729::kan			PMID:16738554	CGSC:101574
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1126	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAGCCAGACCGCGTAAGAA Primer 2:CCCAACGCTACTTTGCTCCATCC
2E7	Kohara Phage	Genobase	PMID:3038334
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 15% ^[6]
zcf-117::Tn10	Linked marker	CAG18463 = CGSC7363^[5]	est. P1 cotransduction: 66% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12370	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12370	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002238	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948034	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2273	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003836	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	IntE
Synonyms	e14 prophage; predicted integrase^[1], B1140^[2]^[1], YcfI^[2]^[1], IntE^[2]^[1] , ECK1126, int, JW1126, ycfI, b1140
Product description	e14 prophage; predicted integrase^[2]^[3] e14 integrase, in defective prophage^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003676	nucleic acid binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013087	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011010	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015094	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016177	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008907	integrase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015094	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015094	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0229	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAARPRKNNV SVPNLYPLYS RKVNKVYWRY KHPVTGKFHA LGTNEAEAIA IATEANTRLA EQRTRQILAI SDRIATSKGK AITTSTWLDR YQAIQDDRLK SGDIRLNTYK QKAKPVSLLR ERAGMKLISA VDVRDIAQLL DEYIAAGRPR MAQVVRSVLI DVFKEAQHYG EVPPGYNPAL ATKQPRRKIT RQRLSLEEWK KIFDIADATH RYMGNAMLLA LVTGQRLGDI SRMKFSDIWD DHLHVIQEKT GSKIAIPLSL RLNAINWSLR DVVARCRDYA VSAYLVHFFR STSQAERGAQ VKANTLTMNF SKARDLARID WGEGSPATFH EQRSLSERLY KEQGLDTQKL LGHKTQQQTD RYHDDRGKGW SKVAL
Length	375
Mol. Wt	42.8 kDa
pI	10.4 (calculated)
Extinction coefficient	59,360 - 59,485 (calc based on 14 Y, 7 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	192..369	PF00589 Phage integrase family	PMID:19920124
Domain	1..84	PF09003 Bacteriophage lambda integrase, N-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=intE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129103	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948034	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003836	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P75969	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12370	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12370	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948034	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002238	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2273	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	10	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
intE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1200009..1200049 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1140 (EcoliWiki Page)	NCBI GEO profiles for intE
microarray	GenExpDB:b1140 (EcoliWiki Page)	Summary of data for intE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to intE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12370	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2273	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1140
EcoGene	EcoGene:EG12370	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002238	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003836	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Gallus gallus	ENSGALP00000000268 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S0941	From SHIGELLACYC
E. coli O157	Z1866	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56349
Pfam (EcoliWiki Page)	PF00589 Phage integrase family
Pfam (EcoliWiki Page)	PF09003 Bacteriophage lambda integrase, N-terminal domain
EcoCyc	EcoCyc:EG12370	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12370	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002238	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2273	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003836	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

intE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to intE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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