ihfB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ihfB

Mnemonic

Integration, host factor

Synonyms

ECK0903, b0912, JW0895, himD[1], hip[1], himD, hip

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

20.76 minutes 

MG1655: 963051..963335
<gbrowseImage> name=NC_000913:963051..963335 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 866019..866303
<gbrowseImage> name=NC_012759:866019..866303 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 964250..964534
<gbrowseImage> name=NC_007779:964250..964534 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1016979..1017263
<gbrowseImage> name=NC_010473:1016979..1017263 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

963051

Edman degradation

PMID:9868784[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔihfB (Keio:JW0895)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8917[4]

ihfBE44G,K,V

E44G,K,V

Altered DNA-binding specificity

seeded from UniProt:P0A6Y1

Δhip-1::cat

PMID:3159903[5]

CGSC:90848

ΔihfB735::kan

PMID:16738554[3]

CGSC:101538


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0895

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCACCAAGTCAGAATTGATAGA

Primer 2:CCACCGTAAATATTGGCGCGATC

6D12

Kohara Phage

Genobase

PMID:3038334[7]

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[4]

est. P1 cotransduction: 55% [8]
Synonyms:zbj-1230::Tn10

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[4]

est. P1 cotransduction: 19% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10441

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10441

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000434

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945533

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0436

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003107

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Berlyn, MK (1998) Linkage map of Escherichia coli K-12, edition 10: the traditional map. Microbiol. Mol. Biol. Rev. 62 814-984 PubMed
  2. Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Flamm, EL & Weisberg, RA (1985) Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor. J. Mol. Biol. 183 117-28 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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