{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	hypF
Gene Synonym(s)	ECK2707, b2712, JW5433, hydA^[1], hydA
Product Desc.	hydrogenase maturation protein^[2]^[3] Hydrogenases 1,2,3 accessory protein; required for CN ligand synthesis at the metallocenter; HypF carbamoyltransferase activity forms thiocarbamate at terminal Cys of HypE; monomeric^[4]
Product Synonyms(s)	carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases^[1], B2712^[2]^[1], HydA^[2]^[1], HypF^[2]^[1], carbamoyltransferase^[2]^[1] , ECK2707, hydA, JW5433, b2712
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hydN-hypF^[2], hydA
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

FhlA regulon.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hypF
Mnemonic	Hydrogenase pleiotropic
Synonyms	ECK2707, b2712, JW5433, hydA^[1], hydA
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	61.06 minutes	MG1655: 2835447..2833195 <gbrowseImage> name=NC_000913:2833195..2835447 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2732285..2730033 <gbrowseImage> name=NC_012967:2730033..2732285 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2719007..2721259 <gbrowseImage> name=NC_012759:2719007..2721259 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2836081..2833829 <gbrowseImage> name=NC_007779:2833829..2836081 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2927989..2925737 <gbrowseImage> name=NC_010473:2925737..2927989 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔhypF (Keio:JW5433)	deletion	deletion		PMID:16738554	Shigen
hypFH476A		H476A	Carbamoyl phosphate-dependent ATP hydrolysis activity diminished in vitro but sufficient for wild-type-like phenotype			seeded from UniProt:P30131
hypFR23E,H,K		R23E,H,K	Loss of activity			seeded from UniProt:P30131
hypFR23Q		R23Q	Diminishes activity			seeded from UniProt:P30131
hypFC112A		C112A	Loss of activity			seeded from UniProt:P30131
hypFC162A		C162A	Loss of activity			seeded from UniProt:P30131
hypFH475A		H475A	Loss of activity			seeded from UniProt:P30131
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5433	Plasmid clone	Shigen	PMID:16769691 Status:cloned as old JW Primer 1:GCCGCAAAAAACACATCTTGCGG Primer 2:CCTCCGTTCTGGACTTCACCCGC
9G10	Kohara Phage	Genobase	PMID:3038334
1B2	Kohara Phage	Genobase	PMID:3038334
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[5]	est. P1 cotransduction: 77% ^[6] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
cysI95::Tn10	Linked marker	CAG12173 = CGSC7425^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:cysC95::Tn10
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11551	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11551	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001504	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944963	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1512	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008915	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HypF
Synonyms	carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases^[1], B2712^[2]^[1], HydA^[2]^[1], HypF^[2]^[1], carbamoyltransferase^[2]^[1] , ECK2707, hydA, JW5433, b2712
Product description	hydrogenase maturation protein^[2]^[3] Hydrogenases 1,2,3 accessory protein; required for CN ligand synthesis at the metallocenter; HypF carbamoyltransferase activity forms thiocarbamate at terminal Cys of HypE; monomeric^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011125	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030528	transcription regulator activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004421	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpmB	PMID:19402753	LCMS(ID Probability):99.6
Protein	gapA	PMID:19402753	LCMS(ID Probability):99.6
Protein	hybC	PMID:19402753	LCMS(ID Probability):99.6
Protein	hybG	PMID:19402753	LCMS(ID Probability):99.6
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAKNTSCGVQ LRIRGKVQGV GFRPFVWQLA QQLNLHGDVC NDGDGVEVRL REDPETFLVQ LYQHCPPLAR IDSVEREPFI WSQLPTEFTI RQSTGGTMNT QIVPDAATCP ACLAEMNTPG ERRYRYPFIN CTHCGPRFTI IRAMPYDRPF TVMAAFPLCP ACDKEYRDPL DRRFHAQPVA CPECGPHLEW VSHGEHAEQE AALQAAIAQL KMGKIVAIKG IGGFHLACDA RNSNAVATLR ARKHRPAKPL AVMLPVADGL PDAARQLLTT PAAPIVLVDK KYVPELCDDI APDLNEVGVM LPANPLQHLL LQELQCPLVM TSGNLSGKPP AISNEQALAD LQGIADGFLI HNRDIVQRMD DSVVRESGEM LRRSRGYVPD ALALPPGFKN VPPVLCLGAD LKNTFCLVRG EQAVLSQHLG DLSDDGIQMQ WREALRLMQN IYDFTPQYVV HDAHPGYVSS QWAREMNLPT QTVLHHHAHA AACLAEHQWP LDGGDVIALT LDGIGMGENG ALWGGECLRV NYRECEHLGG LPAVALPGGD LAAKQPWRNL LAQCLRFVPE WQNYSETASV QQQNWSVLAR AIERGINAPL ASSCGRFFDA VAAALGCAPA TLSYEGEAAC ALEALAASCH GVTHPVTMPR VDNQLDLATF WQQWLNWQAP VNQRAWAFHD ALAQGFAALM REQATMRGIT TLVFSGGVIH NRLLRARLAH YLADFTLLFP QSLPAGDGGL SLGQGVIAAA RWLAGEVQNG
Length	750
Mol. Wt	82.065 kDa
pI	6.4 (calculated)
Extinction coefficient	103,360 - 106,360 (calc based on 14 Y, 15 W, and 24 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	1..91	PF00708 Acylphosphatase	PMID:19920124
Domain	208..374	PF01300 yrdC domain	PMID:19920124
Domain	108..142	PF07503 HypF finger	PMID:19920124
Domain	158..192	PF07503 HypF finger	PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hypF taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130619	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944963	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008915	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P30131	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11551	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11551	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944963	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001504	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1512	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	38	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	14	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	15^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hydN-hypF
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:2835427..2835467 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2712 (EcoliWiki Page)	NCBI GEO profiles for hypF
microarray	GenExpDB:b2712 (EcoliWiki Page)	Summary of data for hypF from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to hypF Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11551	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1512	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2712
EcoGene	EcoGene:EG11551	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001504	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008915	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	HYPF	From SHIGELLACYC
E. coli O157	HYPF	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00708 Acylphosphatase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
Panther (EcoliWiki Page)	PTHR10029:SF4
Superfamily (EcoliWiki Page)	SUPERFAMILY:54975
Superfamily (EcoliWiki Page)	SUPERFAMILY:55821
Pfam (EcoliWiki Page)	PF01300 yrdC domain
Pfam (EcoliWiki Page)	PF07503 HypF finger
Pfam (EcoliWiki Page)	PF07503 HypF finger
EcoCyc	EcoCyc:EG11551	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11551	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001504	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1512	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008915	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hypF:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hypF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search