hyaA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hyaA

Mnemonic

Hydrogenase 1

Synonyms

ECK0963, b0972, JW0954[1], JW0954

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

22.23 minutes 

MG1655: 1031362..1032480
<gbrowseImage> name=NC_000913:1031362..1032480 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1049230..1050348
<gbrowseImage> name=NC_012967:1049230..1050348 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 934330..935448
<gbrowseImage> name=NC_012759:934330..935448 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1032561..1033679
<gbrowseImage> name=NC_007779:1032561..1033679 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1085290..1086408
<gbrowseImage> name=NC_010473:1085290..1086408 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1031497

Edman degradation

PMID:2180913[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhyaA (Keio:JW0954)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8950[4]

hyaA::Tn5KAN-2 (FB20074)

Insertion at nt 834 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20074

does not contain pKD46

ΔhyaA786::kan

PMID:16738554[3]

CGSC:101444


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0954

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAATAACGAGGAAACATTTTA

Primer 2:CCTGCCTGTTTATCCTCATTGCC

4H9

Kohara Phage

Genobase

PMID:3038334[7]

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[4]

est. P1 cotransduction: 32% [8]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[4]

est. P1 cotransduction: 29% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10468

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10468

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000461

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945579

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0463

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003286

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Menon, NK et al. (1990) Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames. J. Bacteriol. 172 1969-77 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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