holC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

holC

Mnemonic

Holoenzyme

Synonyms

ECK4252, b4259, JW4216[1], JW4216


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

96.6 minutes 

MG1655: 4482303..4481860
<gbrowseImage> name=NC_000913:4481860..4482303 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4467313..4466870
<gbrowseImage> name=NC_012967:4466870..4467313 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4420595..4421038
<gbrowseImage> name=NC_012759:4420595..4421038 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4488960..4488517
<gbrowseImage> name=NC_007779:4488517..4488960 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4584111..4583668
<gbrowseImage> name=NC_010473:4583668..4584111 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4481860

Edman degradation

PMID:8246893[2]
PMID:8389364[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔholC (Keio:JW4216)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC11033[5]

ΔholC732::kan

PMID:16738554[4]

CGSC:101400



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4216

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAAACGCGACGTTCTACCT

Primer 2:CCTTTCCAGGTTGCCGTATTCAG

E1F5

Kohara Phage

Genobase

PMID:3038334[7]

5C4

Kohara Phage

Genobase

PMID:3038334[7]

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[5]

est. P1 cotransduction: 22% [8]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[5]

est. P1 cotransduction: % [8]
Synonyms:zjj-202::Tn10, zji-202::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11413

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11413

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001379

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948787

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1385

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013948

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Carter, JR et al. (1993) Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli. Mol. Gen. Genet. 241 399-408 PubMed
  3. ↑ Xiao, H et al. (1993) DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi. J. Biol. Chem. 268 11773-8 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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