holC:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

holC

Mnemonic

Holoenzyme

Synonyms

ECK4252, b4259, JW4216[1], JW4216

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

96.6 minutes 

MG1655: 4482303..4481860
<gbrowseImage> name=NC_000913:4481860..4482303 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4467313..4466870
<gbrowseImage> name=NC_012967:4466870..4467313 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4420595..4421038
<gbrowseImage> name=NC_012759:4420595..4421038 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4488960..4488517
<gbrowseImage> name=NC_007779:4488517..4488960 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4584111..4583668
<gbrowseImage> name=NC_010473:4583668..4584111 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4481860

Edman degradation

PMID:8246893[2]
PMID:8389364[3]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔholC (Keio:JW4216)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC11033[5]

ΔholC732::kan

PMID:16738554[4]

CGSC:101400


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4216

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAAACGCGACGTTCTACCT

Primer 2:CCTTTCCAGGTTGCCGTATTCAG

E1F5

Kohara Phage

Genobase

PMID:3038334[7]

5C4

Kohara Phage

Genobase

PMID:3038334[7]

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[5]

est. P1 cotransduction: 22% [8]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[5]

est. P1 cotransduction: % [8]
Synonyms:zjj-202::Tn10, zji-202::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11413

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11413

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001379

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948787

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1385

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013948

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Carter, JR et al. (1993) Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli. Mol. Gen. Genet. 241 399-408 PubMed
  3. Xiao, H et al. (1993) DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi. J. Biol. Chem. 268 11773-8 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories