hisG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hisG

Mnemonic

Histidine

Synonyms

ECK2014, b2019, JW2001[1], JW2001

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

45.01 minutes 

MG1655: 2088216..2089115
<gbrowseImage> name=NC_000913:2088216..2089115 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2020194..2021093
<gbrowseImage> name=NC_012967:2020194..2021093 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1980699..1981598
<gbrowseImage> name=NC_012759:1980699..1981598 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2092329..2093228
<gbrowseImage> name=NC_007779:2092329..2093228 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2179224..2180123
<gbrowseImage> name=NC_010473:2179224..2180123 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhisG (Keio:JW2001)

deletion

deletion

Auxotrophies

Requires histidine for growth

PMID:16738554[2]

Shigen

CGSC9647[3]

hisG::Tn5KAN-2 (FB20583)

Insertion at position 466 in Minus orientation, contains pKD46

Auxotrophies

Requires Histidine for growth

PMID:15262929[4]

E. coli Genome Project FB20575

hisG::Tn5KAN-2 (FB20583)

Insertion at position 466 in Minus orientation, does not contain pKD46

Auxotrophies

Requires Histidine for growth

PMID:15262929[4]

E. coli Genome Project FB20576

hisG4(Oc)

Auxotrophies

Requires Histidine for growth

PMID:5324068[5] PMID:6450870[6]

CGSC:4501

ochre (UAA) mutation

hisG1(Fs)

Auxotrophies

requires Histidine for growth

PMID:4934197[7]

CGSC:4886

can't find allele in the reference provided

hisG213::Tn10

Auxotrophies

Requires Histidine for growth

CGSC:8789

ΔhisG788::kan

deletion

deletion

Auxotrophies

Requires Histidine for growth

PMID:16738554[2]

CGSC:101370

hisG::Tn5KAN-2 (FB20576)

Insertion at nt 466 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20576

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2001

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCACAGACAACACTCGTTTACG

Primer 2:CCtTCCATCATCTTCTCAATCGG

6D9

Kohara Phage

Genobase

PMID:3038334[9]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[3]

est. P1 cotransduction: 85% [10]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: % [10]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10449

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10449

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000442

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946549

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0444

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006713

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Eggertsson, G & Adelberg, EA (1965) Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics 52 319-40 PubMed
  6. Kato, T et al. (1980) Analysis of ultraviolet light-induced suppressor mutations in the strain of Escherichia coli K-12 AB1157: an implication for molecular mechanisms of UV mutagenesis. Mol. Gen. Genet. 180 283-91 PubMed
  7. Goldschmidt, EP et al. (1970) Genetic analysis of the histidine operon in Escherichia coli K12. Genetics 66 219-29 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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