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Expression|

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Standard Name	hflK
Gene Synonym(s)	ECK4170, b4174, JW4132, hflA, hslY^[1], hslY
Product Desc.	regulator of FtsH protease^[2]^[3]; Component of HflK-HflC complex; regulator of FtsH protease^[3]; HflB, integral membrane ATP-dependent zinc metallopeptidase^[3] Membrane bound protease complex with FtsH and HflC; regulates lysogeny; non-essential^[4]
Product Synonyms(s)	modulator for HflB protease specific for phage lambda cII repressor^[1], B4174^[2]^[1], HslY^[2]^[1], HflA^[2]^[1], HflK^[2]^[1] , ECK4170, hflA, hslY, JW4132, b4174
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yjeFE-amiB-mutL-miaA-hfq-hflXKC^[2], ORF55-ORF17-amiB-mutL-miaA-hfq-hflXKC
Regulation/Activity
Quick Links
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Notes

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Gain-of-function alleles in hflC and hflK inhibit FtsH-mediated degradation of uncomplexed SecY protein and other membrane protein substrates. Null hflC or hflK alleles stabilize cytoplasmic substrate cII. hflCK is a multicopy suppressor of ftsH htpX double mutant. HflC and HflK have a periplasmic prohibitin homology (PHB) domain.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hflK
Mnemonic	High frequency lysogenization
Synonyms	ECK4170, b4174, JW4132, hflA, hslY^[1], hslY
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.84 minutes	MG1655: 4400061..4401320 <gbrowseImage> name=NC_000913:4400061..4401320 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4380193..4381452 <gbrowseImage> name=NC_012967:4380193..4381452 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4338796..4340055 <gbrowseImage> name=NC_012759:4338796..4340055 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4406718..4407977 <gbrowseImage> name=NC_007779:4406718..4407977 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4500423..4501682 <gbrowseImage> name=NC_010473:4500423..4501682 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔhflK (Keio:JW4132)	deletion	deletion	PMID:16738554	Shigen CGSC10975^[5]
ΔhflK724::kan			PMID:16738554	CGSC:101350
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4132	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCGTGGAATCAGCCCGGTAA Primer 2:CCTTCCCCCTGACGCTGGTAGTC
3A1	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 15% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 37% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10436	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10436	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000429	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948698	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0431	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013663	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HflK
Synonyms	modulator for HflB protease specific for phage lambda cII repressor^[1], B4174^[2]^[1], HslY^[2]^[1], HflA^[2]^[1], HflK^[2]^[1] , ECK4170, hflA, hslY, JW4132, b4174
Product description	regulator of FtsH protease^[2]^[3]; Component of HflK-HflC complex; regulator of FtsH protease^[3]; HflB, integral membrane ATP-dependent zinc metallopeptidase^[3] Membrane bound protease complex with FtsH and HflC; regulates lysogeny; non-essential^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010201	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001972	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010201	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009408	response to heat	PMID:8349564	IEP: Inferred from Expression Pattern		P		complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of HflK-HflC complex; regulator of FtsH protease	could be indirect
Protein	gpmA		PMID:19402753	MALDI(Z-score):29.824466
Protein	Subunits of HflB, integral membrane ATP-dependent zinc metallopeptidase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAWNQPGNNG QDRDPWGSSK PGGNSEGNGN KGGRDQGPPD LDDIFRKLSK KLGGLGGGKG TGSGGGSSSQ GPRPQLGGRV VTIAAAAIVI IWAASGFYTI KEAERGVVTR FGKFSHLVEP GLNWKPTFID EVKPVNVEAV RELAASGVML TSDENVVRVE MNVQYRVTNP EKYLYSVTSP DDSLRQATDS ALRGVIGKYT MDRILTEGRT VIRSDTQREL EETIRPYDMG ITLLDVNFQA ARPPEEVKAA FDDAIAAREN EQQYIREAEA YTNEVQPRAN GQAQRILEEA RAYKAQTILE AQGEVARFAK LLPEYKAAPE ITRERLYIET MEKVLGNTRK VLVNDKGGNL MVLPLDQMLK GGNAPAAKSD NGASNLLRLP PASSSTTSGA SNTSSTSQGD IMDQRRANAQ RNDYQRQGE
Length	419
Mol. Wt	45.545 kDa
pI	6.3 (calculated)
Extinction coefficient	39,880 (calc based on 12 Y, 4 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	98..272	PF01145 SPFH domain / Band 7 family	PMID:19920124
Domain	1..60	PF12221 Bacterial membrane protein N terminal	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hflK taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131996	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948698	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013663	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABC7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10436	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10436	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948698	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000429	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0431	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	4.27E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	67.602+/-0.393	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.246227164	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	3846	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1048	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2306	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yjeFE-amiB-mutL-miaA-hfq-hflXKC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4400041..4400081 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4174 (EcoliWiki Page)	NCBI GEO profiles for hflK
microarray	GenExpDB:b4174 (EcoliWiki Page)	Summary of data for hflK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hflK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10436	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0431	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4174
EcoGene	EcoGene:EG10436	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000429	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013663	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	HFLK	From SHIGELLACYC
E. coli O157	HFLK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01145 SPFH domain / Band 7 family
Panther (EcoliWiki Page)	PTHR10264:SF19
Superfamily (EcoliWiki Page)	SUPERFAMILY:117892
Pfam (EcoliWiki Page)	PF12221 Bacterial membrane protein N terminal
EcoCyc	EcoCyc:EG10436	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10436	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000429	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0431	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013663	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hflK:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hflK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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