hemB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hemB

Mnemonic

Hemin

Synonyms

ECK0366, b0369, JW0361, ncf[1], ncf

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

8.36 minutes 

MG1655: 388951..387977
<gbrowseImage> name=NC_000913:387977..388951 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 358635..357661
<gbrowseImage> name=NC_012967:357661..358635 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 290736..291710
<gbrowseImage> name=NC_012759:290736..291710 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 388951..387977
<gbrowseImage> name=NC_007779:387977..388951 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 328282..327308
<gbrowseImage> name=NC_010473:327308..328282 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

387980

Edman degradation

PMID:8439296[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

hemB3

CGSC:11612

hemB1

PMID:1987138[3]

CGSC:11608

hemB220

CGSC:23022

hemB-201

Auxotrophies

δ-aminolevulinate auxotrophy

PMID:1987138[3]

Experimental strain: GE1360 Parental strain: K12

δ-aminolevulinate auxotrophy is usually associated with hemA mutants.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0361

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCACAGACTTAATCCAACGCCC

Primer 2:CCACGCAGAATCTTCTTCTCAGC

8F10

Kohara Phage

Genobase

PMID:3038334[5]

6E2

Kohara Phage

Genobase

PMID:3038334[5]

mhpC281::Tn10

Linked marker

CAG12080 = CGSC7333[6]

est. P1 cotransduction: 55% [7]
Synonyms:zah-281::Tn10

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[6]

est. P1 cotransduction: 81% [7]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10428

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10428

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000421

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945017

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0423

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001266

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Spencer, P & Jordan, PM (1993) Purification and characterization of 5-aminolaevulinic acid dehydratase from Escherichia coli and a study of the reactive thiols at the metal-binding domain. Biochem. J. 290 ( Pt 1) 279-87 PubMed
  3. 3.0 3.1 O'Neill, GP et al. (1991) delta-Aminolevulinic acid dehydratase deficiency can cause delta-aminolevulinate auxotrophy in Escherichia coli. J. Bacteriol. 173 94-100 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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