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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	hcp
Gene Synonym(s)	ECK0864, b0873, JW0857, ybjW, priS^[1]
Product Desc.	hybrid-cluster protein^[2]^[3] Hydroxylamine reductase, hybrid-cluster [2Fe-2S] protein ^[4]
Product Synonyms(s)	hybrid-cluster [4Fe-2S-2O] protein in anaerobic terminal reductases^[1], Hcp^[2]^[1], B0873^[2]^[1], YbjW^[2]^[1], PriS^[2]^[1], fuscoredoxin^[2]^[1], prismane protein^[2]^[1] , ECK0864, JW0857, ybjW, b0873
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hcp-hcr^[2]
Regulation/Activity
Quick Links
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Notes

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Hcp is in an operon and associated with the Hcr oxidoreductase. It is not known if Hcp is a physiologically relevant substrate but Hcp can degrade it in vivo. Hcp is also called fuscoredoxin or prismane protein. Hcp is expressed anaerobically with nitrate or nitrite as terminal electron acceptors and may be involved in metabolizing byproducts of nitrogen metabolism, which might include hydroxylamine. Alternatively is coudl be a catbolic enzyme for an exogeneous substrate, including hydroxylamine. Hcp is upregulated by GSNO in defined medium. FNR regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hcp
Mnemonic	Hybrid-cluster protein
Synonyms	ECK0864, b0873, JW0857, ybjW, priS^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	19.64 minutes	MG1655: 913037..911385 <gbrowseImage> name=NC_000913:911385..913037 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 930521..928869 <gbrowseImage> name=NC_012967:928869..930521 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 814353..816005 <gbrowseImage> name=NC_012759:814353..816005 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 914236..912584 <gbrowseImage> name=NC_007779:912584..914236 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 966965..965313 <gbrowseImage> name=NC_010473:965313..966965 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δhcp (Keio:JW0857)	deletion	deletion	PMID:16738554	Shigen CGSC8890^[5]
Δhcp-774::kan			PMID:16738554	CGSC:101322
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0857	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTTTGTGTGCAATGTGAACA Primer 2:CCCGCGCTCAACAGTTGCTTCAT
14H6	Kohara Phage	Genobase	PMID:3038334
1E5	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 24% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003057	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946592	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3456	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Hcp
Synonyms	hybrid-cluster [4Fe-2S-2O] protein in anaerobic terminal reductases^[1], Hcp^[2]^[1], B0873^[2]^[1], YbjW^[2]^[1], PriS^[2]^[1], fuscoredoxin^[2]^[1], prismane protein^[2]^[1] , ECK0864, JW0857, ybjW, b0873
Product description	hybrid-cluster protein^[2]^[3] Hydroxylamine reductase, hybrid-cluster [2Fe-2S] protein ^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0016491	oxidoreductase activity	PMID:16928682	IDA: Inferred from Direct Assay		F		complete
	GO:0055114	oxidation reduction	PMID:16928682	IMP: Inferred from Mutant Phenotype		P	Figure 2 & 3.	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011254	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016099	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003824	catalytic activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016100	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00069	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004137	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010048	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004137	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042542	response to hydrogen peroxide	PMID:16928682	IEP: Inferred from Expression Pattern		P	Figure 1	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004601	peroxidase activity	PMID:16928682	IDA: Inferred from Direct Assay		F	Figure 4.	complete
	GO:0016661	oxidoreductase activity, acting on other nitrogenous compounds as donors	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010048	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0050418	hydroxylamine reductase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00069	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010048	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051536	iron-sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0411	F	Seeded from EcoCyc (v14.0)	complete
	GO:0051537	2 iron, 2 sulfur cluster binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0001	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004137	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010048	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016099	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016100	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	poxB		PMID:16606699	Experiment(s):EBI-1138223
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIMFCVQCEQ TIRTPAGNGC SYAQGMCGKT AETSDLQDLL IAALQGLSAW AVKAREYGII NHDVDSFAPR AFFSTLTNVN FDSPRIVGYA REAIALREAL KAQCLAVDAN ARVDNPMADL QLVSDDLGEL QRQAAEFTPN KDKAAIGENI LGLRLLCLYG LKGAAAYMEH AHVLGQYDND IYAQYHKIMA WLGTWPADMN ALLECSMEIG QMNFKVMSIL DAGETGKYGH PTPTQVNVKA TAGKCILISG HDLKDLYNLL EQTEGTGVNV YTHGEMLPAH GYPELRKFKH LVGNYGSGWQ NQQVEFARFP GPIVMTSNCI IDPTVGAYDD RIWTRSIVGW PGVRHLDGDD FSAVITQAQQ MAGFPYSEIP HLITVGFGRQ TLLGAADTLI DLVSREKLRH IFLLGGCDGA RGERHYFTDF ATSVPDDCLI LTLACGKYRF NKLEFGDIEG LPRLVDAGQC NDAYSAIILA VTLAEKLGCG VNDLPLSLVL SWFEQKAIVI LLTLLSLGVK NIVTGPTAPG FLTPDLLAVL NEKFGLRSIT TVEEDMKQLL SA
Length	552
Mol. Wt	60.309 kDa
pI	5.1 (calculated)
Extinction coefficient	65,320 - 67,070 (calc based on 18 Y, 7 W, and 14 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..548	PF03063 Prismane/CO dehydrogenase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hcp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111180	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946592	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002962	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P75825	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13692	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946592	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003057	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3456	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hcp-hcr
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:913017..913057 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0873 (EcoliWiki Page)	NCBI GEO profiles for hcp
microarray	GenExpDB:b0873 (EcoliWiki Page)	Summary of data for hcp from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (912946..913183) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ07; Well:C7^[7]
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Notes

Accessions Related to hcp Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6457	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3456	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0873
EcoGene	EcoGene:EG13692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003057	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YBJW	From SHIGELLACYC
E. coli O157	YBJW	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56821
Pfam (EcoliWiki Page)	PF03063 Prismane/CO dehydrogenase family
EcoCyc	EcoCyc:G6457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003057	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3456	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hcp:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hcp Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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