{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	gspB
Gene Synonym(s)	pioO, ECK3309, b3322, JW3284, pinO^[1]^[2], pinO
Product Desc.	calcium-binding protein required for initiation of chromosome replication^[3]^[2] Part of H-NS-silenced gsp divergon, type II protein secretion; cloned gsp divergon secretes ChiA, requires gspAB operon^[4]
Product Synonyms(s)	part of gsp divergon involved in type II protein secretion^[1], B3322^[3]^[1], GspB^[3]^[1], PioO^[3]^[1], PinO^[3]^[1] , ECK3309, JW3284, pinO, pioO, b3322
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): pioO^[3], gspA-pioO^[3]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

GspB is a distant homolog or convergent analog of exeB of A. hydrophila, which is required for protein export , specifically for the export and assembly of secretin (ExeD/GspD) into the outer membrane. GspB has a single predicted N-terminal transmembrane segment (28-50), followed by a predicted periplasmic domain, which may interact with GspA. GspA may interact with GspB. A divergon is a regulon comprised of two adjacent divergent operons or genes. gspB was initially identified as an ORF that might be the 11.3 kDa PinO protein synthesized in the absence of isoleucine in relA stains (Guzman, 1991). But GspB is predicted to be 15.9 kDa and has isoleucine residues and there is no evidence that gspB encodes the PinO peptide or that it binds calcium. No homology to calmodulin or other calcium-binding proteins is predicted. gspB may have been mistaken for pinO due to use of a downstream ATG as start codon, but the 11 kD C-terminal fragment does have an Ile reside in it, making gspB inconsistent with pinO. pioO was the prior gene name assigned to pinO since the gene name pin was already taken.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gspB
Mnemonic	General secretory pathway
Synonyms	pioO, ECK3309, b3322, JW3284, pinO^[1]^[2], pinO
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	74.39 minutes	MG1655: 3451949..3451530 <gbrowseImage> name=NC_000913:3451530..3451949 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3381413..3381832 <gbrowseImage> name=NC_012967:3381413..3381832 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3338687..3339106 <gbrowseImage> name=NC_012759:3338687..3339106 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4186489..4186908 <gbrowseImage> name=NC_007779:4186489..4186908 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
pinO::Tn5KAN-I-SceI (FB21106)	Insertion at nt 176 in Minus orientation				PMID:15262929	E. coli Genome Project:FB21106	does not contain pKD46
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
9H3	Kohara Phage	Genobase	PMID:3038334
E1H9	Kohara Phage	Genobase	PMID:3038334
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11263	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11263	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001238	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947826	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1243	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010865	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	PioO
Synonyms	part of gsp divergon involved in type II protein secretion^[1], B3322^[3]^[1], GspB^[3]^[1], PioO^[3]^[1], PinO^[3]^[1] , ECK3309, JW3284, pinO, pioO, b3322
Product description	calcium-binding protein required for initiation of chromosome replication^[3]^[2] Part of H-NS-silenced gsp divergon, type II protein secretion; cloned gsp divergon secretes ChiA, requires gspAB operon^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005509	calcium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0106	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006260	DNA replication	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0235	P	Seeded from EcoCyc (v14.0)	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFEFYIAARE QKETGHPGIF SRQKHSTIIY VICLLLICLW FAGMVLVGGY ARQLWVLWIV KAEVTVEAET PAFKQSTQHY FFKKQPLPVV ESVEEEDDPG VAVENAPSSS EDEENTVEES EEKAGLRERV KNALNELER
Length	139
Mol. Wt	15.875 kDa
pI	4.7 (calculated)
Extinction coefficient	22,460 - 22,710 (calc based on 4 Y, 3 W, and 2 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gspB taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131201	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947826	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010865	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P03825	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11263	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11263	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947826	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001238	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1243	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
pioO
gspA-pioO
edit table

File:.jpg

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:3451929..3451969 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3322 (EcoliWiki Page)	NCBI GEO profiles for gspB
microarray	GenExpDB:b3322 (EcoliWiki Page)	Summary of data for gspB from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to gspB Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11263	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1243	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3322
EcoGene	EcoGene:EG11263	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001238	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010865	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
edit table

</protect>

Do-It-Yourself Web Tools

Notes

Families

[back to top]

Database	Accession	Notes
PFAM (EcoliWiki Page)	PFAM:PF00338
edit table

Notes

Families

[back to top]

<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
edit table

</protect> <protect></protect>

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

gspB:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gspB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search