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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	gmk
Gene Synonym(s)	ECK3638, b3648, JW3623, spoR^[1], spoR
Product Desc.	Gmk^[2]^[3]; Component of GUANYL-KIN-CPLX^[2] Guanylate kinase^[4]
Product Synonyms(s)	guanylate kinase^[1], B3648^[2]^[1], SpoR^[2]^[1], Gmk^[2]^[1] , ECK3638, JW3623, spoR, b3648
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gmk^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gmk
Mnemonic	GMP kinase
Synonyms	ECK3638, b3648, JW3623, spoR^[1], spoR
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	82.32 minutes	MG1655: 3819451..3820074 <gbrowseImage> name=NC_000913:3819451..3820074 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3759785..3760408 <gbrowseImage> name=NC_012967:3759785..3760408 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3707778..3708401 <gbrowseImage> name=NC_012759:3707778..3708401 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3818987..3818364 <gbrowseImage> name=NC_007779:3818364..3818987 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3917029..3917652 <gbrowseImage> name=NC_010473:3917029..3917652 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3623	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTCAAGGCACGCTTTATAT Primer 2:CCGTCTGCCAACAATTTGCTGAT
7F3	Kohara Phage	Genobase	PMID:3038334
2A6	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 76% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10965	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000954	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948163	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0958	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011930	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Gmk
Synonyms	guanylate kinase^[1], B3648^[2]^[1], SpoR^[2]^[1], Gmk^[2]^[1] , ECK3638, JW3623, spoR, b3648
Product description	Gmk^[2]^[3]; Component of GUANYL-KIN-CPLX^[2] Guanylate kinase^[4]
EC number (for enzymes)	2.7.4.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004385	guanylate kinase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00328	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004385	guanylate kinase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017665	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004385	guanylate kinase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.7.4.8	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00328	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00328	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006163	purine nucleotide metabolic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00328	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006163	purine nucleotide metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017665	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of GUANYL-KIN-CPLX	could be indirect
Protein	ybfD		PMID:16606699	Experiment(s):EBI-1146400
Protein	rpsI		PMID:16606699	Experiment(s):EBI-1146400
Protein	ybcK		PMID:16606699	Experiment(s):EBI-1146400
Protein	ilvC		PMID:16606699	Experiment(s):EBI-1146400
Protein	rplP		PMID:16606699	Experiment(s):EBI-1146400
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAQGTLYIVS APSGAGKSSL IQALLKTQPL YDTQVSVSHT TRQPRPGEVH GEHYFFVNHD EFKEMISRDA FLEHAEVFGN YYGTSREAIE QVLATGVDVF LDIDWQGAQQ IRQKMPHARS IFILPPSKIE LDRRLRGRGQ DSEEVIAKRM AQAVAEMSHY AEYDYLIVND DFDTALTDLK TIIRAERLRM SRQKQRHDAL ISKLLAD
Length	207
Mol. Wt	23.592 kDa
pI	6.5 (calculated)
Extinction coefficient	17,420 (calc based on 8 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..186	PF00625 Guanylate kinase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gmk taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131519	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948163	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011930	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P60546	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10965	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10965	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948163	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000954	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0958	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	8.35E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	39.269+/-0.162	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.209470305	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	4423	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	524	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1537	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gmk
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3819431..3819471 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3648 (EcoliWiki Page)	NCBI GEO profiles for gmk
microarray	GenExpDB:b3648 (EcoliWiki Page)	Summary of data for gmk from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3818734..3819098) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:E9^[7]
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Notes

Accessions Related to gmk Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10965	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0958	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3648
EcoGene	EcoGene:EG10965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000954	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011930	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000012655 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000026035 (score: 1.000; bootstrap: 94%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G41880 (score: 1.000; bootstrap: 63%) AT3G57550 (score: 0.762)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000036402 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00032989 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00020190 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000001672 (score: 1.000; bootstrap: 89%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000014171 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Danio rerio	ZDB-CDNA-040425-2625 (score: 1.000; bootstrap: 97%) ZDB-CDNA-040425-2624 (score: 0.942) ZDB-GENE-020916-1 (score: 0.942) ZDB-CDNA-040625-191 (score: 0.736) ZDB-GENE-040625-130 (score: 0.736)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230100 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0036099 (score: 1.000; bootstrap: 98%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA11213-PA (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000030670 (score: 1.000; bootstrap: 90%)	From Inparanoid:20070104
Homo sapiens	ENSP00000317659 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000030393 (score: 1.000; bootstrap: 95%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000017243 (score: 1.000; bootstrap: 96%)	From Inparanoid:20070104
Mus musculus	MGI:95871 (score: 1.000; bootstrap: 98%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000003496 (score: 1.000; bootstrap: 92%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000003926 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR454C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC11985 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000150429 (score: 1.000; bootstrap: 94%) NEWSINFRUP00000156898 (score: 0.644)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00028903001 (score: 1.000; bootstrap: 90%) GSTENP00016152001 (score: 0.400)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000030432 (score: 1.000; bootstrap: 60%)	From Inparanoid:20070104
Shigella flexneri	GMK	From SHIGELLACYC
E. coli O157	GMK	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00625 Guanylate kinase
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR23117:SF1
EcoCyc	EcoCyc:EG10965	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10965	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000954	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0958	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011930	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

gmk:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gmk Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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