glyW:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glyW

Mnemonic

Glycine

Synonyms

ECK1910, b1911, JWR0035, ins, mutC, suA58, suA78, G289[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

42.89 minutes 

MG1655: 1990141..1990066
<gbrowseImage> name=NC_000913:1990066..1990141 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1970035..1970110
<gbrowseImage> name=NC_012967:1970035..1970110 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1882549..1882624
<gbrowseImage> name=NC_012759:1882549..1882624 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1994254..1994179
<gbrowseImage> name=NC_007779:1994179..1994254 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2081149..2081074
<gbrowseImage> name=NC_010473:2081074..2081149 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mutC

C35T

mutator

mutator

PMID:8633075[3]

Changes the anticodon to read GAU/C aspartate codons. Thought to be a mutator by virtue of rare mistranslation of proteins involved in DNA fidelity.

mutC

C35T

UVM-constitutive phenotype

PMID:9393717[4]

Changes the anticodon to read GAU/C aspartate codons.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:

18A9

Kohara Phage

Genobase

PMID:3038334[6]

16B12

Kohara Phage

Genobase

PMID:3038334[6]

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[7]

est. P1 cotransduction: 4% [8]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[7]

est. P1 cotransduction: 99% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG30039

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG30039

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002481

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946416

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4202

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006365

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Slupska, MM et al. (1996) Mutator tRNAs are encoded by the Escherichia coli mutator genes mutA and mutC: a novel pathway for mutagenesis. Proc. Natl. Acad. Sci. U.S.A. 93 4380-5 PubMed
  4. Murphy, HS & Humayun, MZ (1997) Escherichia coli cells expressing a mutant glyV (glycine tRNA) gene have a UVM-constitutive phenotype: implications for mechanisms underlying the mutA or mutC mutator effect. J. Bacteriol. 179 7507-14 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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