gltB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

gltB

Gene Synonym(s)

ECK3202, b3212, JW3179, aspB, ossB, psiQ[1], psiQ

Product Desc.

glutamate synthase (NADPH) large chain precursor[2]; glutamate synthase, large subunit[3];

Component of glutamate synthase[2][3]; glutamate synthase (NADPH)[2]

Glutamate synthase, large subunit[4]

Product Synonyms(s)

glutamate synthase, large subunit[1], B3212[2][1], OssB[2][1], PsiQ[2][1], AspB[2][1], GltB[2][1] , aspB, ECK3202, JW3179, ossB, psiQ, b3212

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): gltBDF[2], OP00253, gltD

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gltB

Mnemonic

Glutamate

Synonyms

ECK3202, b3212, JW3179, aspB, ossB, psiQ[1], psiQ

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.26 minutes 

MG1655: 3352654..3357207
<gbrowseImage> name=NC_000913:3352654..3357207 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3289867..3294420
<gbrowseImage> name=NC_012967:3289867..3294420 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3239802..3244355
<gbrowseImage> name=NC_012759:3239802..3244355 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3354487..3359040
<gbrowseImage> name=NC_007779:3354487..3359040 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3450399..3454952
<gbrowseImage> name=NC_010473:3450399..3454952 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔgltB (Keio:JW3179)

deletion

deletion

PMID:16738554

Shigen
CGSC10412[5]

gltB17

PMID:374339

CGSC:8181

gltB13

CGSC:9332

gltB32

CGSC:9793

ΔgltB740::kan

PMID:16738554

CGSC:101198


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3179

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACACGCAAACCCCGTCGCCA

Primer 2:CCtTGCGCCTGCACGCGCAACTC

3G10

Kohara Phage

Genobase

PMID:3038334

8D6

Kohara Phage

Genobase

PMID:3038334

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[5]

est. P1 cotransduction: 46% [6]
Synonyms:zgj-203::Tn10, zha-203::Tn10

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[5]

est. P1 cotransduction: 68% [6]
Synonyms:zha-6::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10403

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10403

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000396

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947724

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0398

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010545

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GltB

Synonyms

glutamate synthase, large subunit[1], B3212[2][1], OssB[2][1], PsiQ[2][1], AspB[2][1], GltB[2][1] , aspB, ECK3202, JW3179, ossB, psiQ, b3212

Product description

glutamate synthase (NADPH) large chain precursor[2]; glutamate synthase, large subunit[3];

Component of glutamate synthase[2][3]; glutamate synthase (NADPH)[2]

Glutamate synthase, large subunit[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004355

glutamate synthase (NADPH) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.4.1.13

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006537

glutamate biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002932

P

Seeded from EcoCyc (v14.0)

complete

GO:0006537

glutamate biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0314

P

Seeded from EcoCyc (v14.0)

complete

GO:0006541

glutamine metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0315

P

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051536

iron-sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0411

F

Seeded from EcoCyc (v14.0)

complete

GO:0051538

3 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0003

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002489

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006982

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of glutamate synthase

could be indirect

Protein

katG

PMID:19402753

LCMS(ID Probability):99.6

Protein

glpK

PMID:19402753

MALDI(Z-score):24.217330

Protein

ebgA

PMID:19402753

MALDI(Z-score):17.480125

Protein

gltD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):25.464654

Protein

Subunits of glutamate synthase (NADPH)

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTRKPRRHAL SVPVRSGSEV GFPQSLGEVH DMLYDKSLER DNCGFGLIAH IEGEPSHKVV
RTAIHALARM QHRGAILADG KTGDGCGLLL QKPDRFFRIV AQERGWRLAK NYAVGMLFLN
KDPELAAAAR RIVEEELQRE TLSIVGWRDV PTNEGVLGEI ALSSLPRIEQ IFVNAPAGWR
PRDMERRLFI ARRRIEKRLE ADKDFYVCSL SNLVNIYKGL CMPTDLPRFY LDLADLRLES
AICLFHQRFS TNTVPRWPLA QPFRYLAHNG EINTITGNRQ WARARTYKFQ TPLIPDLHDA
APFVNETGSD SSSMDNMLEL LLAGGMDIIR AMRLLVPPAW QNNPDMDPEL RAFFDFNSMH
MEPWDGPAGI VMSDGRFAAC NLDRNGLRPA RYVITKDKLI TCASEVGIWD YQPDEVVEKG
RVGPGELMVI DTRSGRILHS AETDDDLKSR HPYKEWMEKN VRRLVPFEDL PDEEVGSREL
DDDTLASYQK QFNYSAEELD SVIRVLGENG QEAVGSMGDD TPFAVLSSQP RIIYDYFRQQ
FAQVTNPPID PLREAHVMSL ATSIGREMNV FCEAEGQAHR LSFKSPILLY SDFKQLTTMK
EEHYRADTLD ITFDVTKTTL EATVKELCDK AEKMVRSGTV LLVLSDRNIA KDRLPVPAPM
AVGAIQTRLV DQSLRCDANI IVETASARDP HHFAVLLGFG ATAIYPYLAY ETLGRLVDTH
AIAKDYRTVM LNYRNGINKG LYKIMSKMGI STIASYRCSK LFEAVGLHDD VVGLCFQGAV
SRIGGASFED FQQDLLNLSK RAWLARKPIS QGGLLKYVHG GEYHAYNPDV VRTLQQAVQS
GEYSDYQEYA KLVNERPATT LRDLLAITPG ENAVNIADVE PASELFKRFD TAAMSIGALS
PEAHEALAEA MNSIGGNSNS GEGGEDPARY GTNKVSRIKQ VASGRFGVTP AYLVNADVIQ
IKVAQGAKPG EGGQLPGDKV TPYIAKLRYS VPGVTLISPP PHHDIYSIED LAQLIFDLKQ
VNPKAMISVK LVSEPGVGTI ATGVAKAYAD LITIAGYDGG TGASPLSSVK YAGCPWELGL
VETQQALVAN GLRHKIRLQV DGGLKTGVDI IKAAILGAES FGFGTGPMVA LGCKYLRICH
LNNCATGVAT QDDKLRKNHY HGLPFKVTNY FEFIARETRE LMAQLGVTRL VDLIGRTDLL
KELDGFTAKQ QKLALSKLLE TAEPHPGKAL YCTENNPPFD NGLLNAQLLQ QAKPFVDERQ
SKTFWFDIRN TDRSVGASLS GYIAQTHGDQ GLAADPIKAY FNGTAGQSFG VWNAGGVELY
LTGDANDYVG KGMAGGLIAI RPPVGSAFRS HEASIIGNTC LYGATGGRLY AAGRAGERFG
VRNSGAITVV EGIGDNGCEY MTGGIVCILG KTGVNFGAGM TGGFAYVLDE SGDFRKRVNP
ELVEVLSVDA LAIHEEHLRG LITEHVQHTG SQRGEEILAN WSTFATKFAL VKPKSSDVKA
LLGHRSRSAA ELRVQAQ
Length

1,517

Mol. Wt

166.713 kDa

pI

6.7 (calculated)

Extinction coefficient

150,010 - 152,510 (calc based on 49 Y, 14 W, and 20 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

485..769

PF04898 Glutamate synthase central domain

PMID:19920124

Domain

1264..1461

PF01493 GXGXG motif

PMID:19920124

Domain

43..406

PF00310 Glutamine amidotransferases class-II

PMID:19920124

Domain

824..1188

PF01645 Conserved region in glutamate synthase

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gltB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:308209621

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947724

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010545

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09831

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10403

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10403

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947724

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000396

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0398

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

7.29E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

174.805+/-0.994

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.10845+/-0.01198

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.362398419

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1079

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

6013

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1004

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

gltBDF

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3352634..3352674 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3212 (EcoliWiki Page)

NCBI GEO profiles for gltB

microarray

GenExpDB:b3212 (EcoliWiki Page)

Summary of data for gltB from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3351589..3352343) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:F7[7]

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Notes

Accessions Related to gltB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10403

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0398

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3212

EcoGene

EcoGene:EG10403

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000396

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010545

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000013025 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000028277 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G04140 (score: 1.000; bootstrap: 68%)
  • AT2G41220 (score: 0.692)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00025132 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00012326 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036663 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21956-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • P23225 (score: 1.000; bootstrap: 100%)
  • Q69RJ0 (score: 0.857)
  • O04187 (score: 0.498)
  • O04186 (score: 0.103)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDL171C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAPB1E77 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

GLTB

From SHIGELLACYC

E. coli O157

GLTB

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00310 Glutamine amidotransferases class-II

Pfam (EcoliWiki Page)

PF01493 GXGXG motif

Pfam (EcoliWiki Page)

PF04898 Glutamate synthase central domain

Pfam (EcoliWiki Page)

PF01645 Conserved region in glutamate synthase

Superfamily (EcoliWiki Page)

SUPERFAMILY:51395

Panther (EcoliWiki Page)

PTHR11938:SF1

Superfamily (EcoliWiki Page)

SUPERFAMILY:56235

Superfamily (EcoliWiki Page)

SUPERFAMILY:69336

EcoCyc

EcoCyc:EG10403

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10403

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000396

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0398

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010545

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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