glpD:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpD

Mnemonic

Glycerol phosphate

Synonyms

ECK3412, b3426, JW3389, glyD, glvD[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.73 minutes 

MG1655: 3560036..3561541
<gbrowseImage> name=NC_000913:3560036..3561541 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3490614..3492119
<gbrowseImage> name=NC_012967:3490614..3492119 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3448530..3450035
<gbrowseImage> name=NC_012759:3448530..3450035 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4078402..4076897
<gbrowseImage> name=NC_007779:4076897..4078402 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3657781..3659286
<gbrowseImage> name=NC_010473:3657781..3659286 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3560036

Edman degradation

PMID:8982270[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpD (Keio:JW3389)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10523[4]

glpD::Tn5KAN-I-SceI (FB21196)

Insertion at nt 1179 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB21196

does not contain pKD46

glpD3

CGSC:4920

glpD11(ts)

temperature sensitive

CGSC:5541

glpD10

CGSC:5656

glpD26

CGSC:6606

glpD19

CGSC:15788

ΔglpD759::kan

PMID:16738554[3]

CGSC:101182


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3389

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGAAACCAAAGATCTGATTGT

Primer 2:CCaGACGCCAGCGATAACCTCTG

E5B4

Kohara Phage

Genobase

PMID:3038334[7]

E4E4

Kohara Phage

Genobase

PMID:3038334[7]

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 6% [8]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[4]

est. P1 cotransduction: 90% [8]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10394

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10394

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000387

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947934

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0389

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011191

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Milla, ME & Raetz, CR (1996) Association of lipid A disaccharide synthase with aerobic glycerol-3-phosphate dehydrogenase in extracts of Escherichia coli. Biochim. Biophys. Acta 1304 245-53 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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