glpC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpC

Mnemonic

Glycerol phosphate

Synonyms

ECK2235, b2243, JW2237[1], JW2237

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

50.73 minutes 

MG1655: 2353543..2354733
<gbrowseImage> name=NC_000913:2353543..2354733 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2302229..2303419
<gbrowseImage> name=NC_012967:2302229..2303419 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2239348..2240538
<gbrowseImage> name=NC_012759:2239348..2240538 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2360191..2361381
<gbrowseImage> name=NC_007779:2360191..2361381 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2444531..2445721
<gbrowseImage> name=NC_010473:2444531..2445721 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2353543

Edman degradation

PMID:3286606[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpC (Keio:JW2237)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9803[4]

glpC::Tn5KAN-2 (FB20727)

Insertion at nt 860 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20727

does not contain pKD46

glpC::Tn5KAN-2 (FB20728)

Insertion at nt 860 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20728

contains pKD46

ΔglpC723::kan

PMID:16738554[3]

CGSC:101180


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2237

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAATGACACCAGCTTCGAAAA

Primer 2:CCAGCCAGCGCCTGGGCCAGTAG

2E8

Kohara Phage

Genobase

PMID:3038334[7]

22C4

Kohara Phage

Genobase

PMID:3038334[7]

4B4

Kohara Phage

Genobase

PMID:3038334[7]

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[4]

est. P1 cotransduction: 70% [8]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[4]

est. P1 cotransduction: 23% [8]
Synonyms:zej-223::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10393

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10393

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000386

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946735

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0388

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007426

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Cole, ST et al. (1988) Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12. J. Bacteriol. 170 2448-56 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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