glpA:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glpA

Mnemonic

Glycerol phosphate

Synonyms

ECK2233, b2241, JW2235[1], JW2235

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

50.66 minutes 

MG1655: 2350669..2352297
<gbrowseImage> name=NC_000913:2350669..2352297 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2299355..2300983
<gbrowseImage> name=NC_012967:2299355..2300983 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2236474..2238102
<gbrowseImage> name=NC_012759:2236474..2238102 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2357317..2358945
<gbrowseImage> name=NC_007779:2357317..2358945 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2441657..2443285
<gbrowseImage> name=NC_010473:2441657..2443285 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2350669

Edman degradation

PMID:3286606[2]


<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglpA (Keio:JW2235)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9801[4]

glpA::Tn5KAN-2 (FB20723)

Insertion at nt 970 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20723

does not contain pKD46

glpA::Tn5KAN-2 (FB20724)

Insertion at nt 970 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20724

contains pKD46

glpA9

CGSC:5628

glpA24

CGSC:6215

glpA25

CGSC:6465

ΔglpA721::kan

PMID:16738554[3]

CGSC:101176


<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2235

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAAACTCGCGACTCGCAATC

Primer 2:CCAAGCGCATCTTTCTGCTCCTT

2E8

Kohara Phage

Genobase

PMID:3038334[7]

22C4

Kohara Phage

Genobase

PMID:3038334[7]

zfa-723::Tn10

Linked marker

CAG12178 gyrA+ = CGSC7526[4]

est. P1 cotransduction: 77% [8]
Synonyms:zei-723::Tn10, zfa-723::Tn10 nnnThe original CAG12178 was NalR. This is a NalS derivative constructed by S. McAteer (CGSC).

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[4]

est. P1 cotransduction: 20% [8]
Synonyms:zej-223::Tn10

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10391

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10391

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000384

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946713

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0386

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007422

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Cole, ST et al. (1988) Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12. J. Bacteriol. 170 2448-56 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories