glcF:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glcF

Mnemonic

Glycolate

Synonyms

ECK2972, b4467, JW5486, b2978, yghL, gox, b2978 (obsolete)[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

67.29 minutes 

MG1655: 3123481..3122258
<gbrowseImage> name=NC_000913:3122258..3123481 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3052351..3051128
<gbrowseImage> name=NC_012967:3051128..3052351 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3009406..3010629
<gbrowseImage> name=NC_012759:3009406..3010629 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3124115..3122892
<gbrowseImage> name=NC_007779:3122892..3124115 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3221226..3220003
<gbrowseImage> name=NC_010473:3220003..3221226 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglcF (Keio:JW5486)

deletion

deletion

PMID:16738554[3]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5486

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCAAACCCAATTAACTGAAGA

Primer 2:CCTTCCTTTTCAAGGGCTTGTTC

1G7

Kohara Phage

Genobase

PMID:3038334[5]

12F3

Kohara Phage

Genobase

PMID:3038334[5]

2F9

Kohara Phage

Genobase

PMID:3038334[5]

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[6]

est. P1 cotransduction: 52% [7]

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[6]

est. P1 cotransduction: 34% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G0-8601

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13291

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002572

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:2847717

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3076

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0174097

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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