gapC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gapC

Mnemonic
Synonyms

Note that this is a pseudogene in K12 strains., ECK1409, JW1413, JW5906, b4493

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

32.07 minutes 

MG1655: 1487988..1487737
<gbrowseImage> name=NC_000913:1487737..1487988 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1491678..1491427
<gbrowseImage> name=NC_007779:1491427..1491678 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

32.07 minutes 

MG1655: 1488737..1487985
<gbrowseImage> name=NC_000913:1487985..1488737 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1492427..1491675
<gbrowseImage> name=NC_007779:1491675..1492427 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

 

MG1655: 1488737..1487737
<gbrowseImage> name=NC_000913:1487737..1488737 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1492427..1491427
<gbrowseImage> name=NC_007779:1491427..1492427 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

gapC_2::Tn5KAN-2 (FB20315)

Insertion at nt 128 in Plus orientation

PMID:15262929[1]

E. coli Genome Project:FB20314

contains pKD46

gapC_2::Tn5KAN-2 (FB20315)

Insertion at nt 128 in Plus orientation

PMID:15262929[1]

E. coli Genome Project:FB20315

does not contain pKD46

ΔgapC777::kan

PMID:16738554[2]

CGSC:101092


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[3]

Status:

Primer 1:

Primer 2:

3C9

Kohara Phage

Genobase

PMID:3038334[4]

24D6

Kohara Phage

Genobase

PMID:3038334[4]

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[5]

est. P1 cotransduction: 7% [6]

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[5]

est. P1 cotransduction: 82% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1417

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GAPC

NCBI (EcoliWiki Page)

GeneID:2847738

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004456

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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