ftsW:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

ftsW

Gene Synonym(s)

ECK0090, b0089, JW0087[1], JW0087

Product Desc.

essential cell division protein FtsW[2][3]

Stabilizes FtsZ ring, membrane protein; faciltates septal petidoglycan synthesis by recruiting the cognate FtsI transpeptidase; SEDS protein[4]

During cell division FtsW is localized at the septum. [5]

Product Synonyms(s)

integral membrane protein involved in stabilizing FstZ ring during cell division[1], B0089[2][1], FtsW[2][1] , ECK0090, JW0087, b0089

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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FtsW is required for mrdA(PBP2) expression. ftsW is paralogous to mrdB(rodA). ftsW is an essential gene. Divisome.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ftsW

Mnemonic

Filamentation, temperature sensitive

Synonyms

ECK0090, b0089, JW0087[1], JW0087

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

2.12 minutes 

MG1655: 98403..99647
<gbrowseImage> name=NC_000913:98403..99647 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 101207..102451
<gbrowseImage> name=NC_012967:101207..102451 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 98402..99646
<gbrowseImage> name=NC_012759:98402..99646 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 98403..99647
<gbrowseImage> name=NC_007779:98403..99647 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 72507..73751
<gbrowseImage> name=NC_010473:72507..73751 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ftsWE170K

E170K

In ftsW201; TS; blocks division at the initiation stage

seeded from UniProt:P0ABG4

ftsWP181L

P181L

In ftsW1640; TS; blocks division at a late stage

seeded from UniProt:P0ABG4

ftsWP253L

P253L

In ftsW263; TS; blocks division at the initiation stage

seeded from UniProt:P0ABG4

ftsW263

C to T

Pro253Leu

Growth Phenotype

blocks division at the initiation stage

PMID:9006034

ftsW1640

C to T

Pro 181 Leu

Growth Phenotype

blocks division at a later stage

PMID:9006034

ftsW201

G to A

Glu 170 Lysine

Growth Phenotype

blocks division at the initiation stage

PMID:7961485 PMID:9006034


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0087

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCGTTTATCTCTCCCTCGCCT

Primer 2:CCTCGTGAACCTCGTACAAACGC

6F3

Kohara Phage

Genobase

PMID:3038334

15B8

Kohara Phage

Genobase

PMID:3038334

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[6]

est. P1 cotransduction: 59% [7]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[6]

est. P1 cotransduction: 10% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10344

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10344

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000338

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946322

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0340

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000320

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FtsW

Synonyms

integral membrane protein involved in stabilizing FstZ ring during cell division[1], B0089[2][1], FtsW[2][1] , ECK0090, JW0087, b0089

Product description

essential cell division protein FtsW[2][3]

Stabilizes FtsZ ring, membrane protein; faciltates septal petidoglycan synthesis by recruiting the cognate FtsI transpeptidase; SEDS protein[4]

During cell division FtsW is localized at the septum. [5]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005515

protein binding

PMID:18165305

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0007047

cellular cell wall organization

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0961

P

Seeded from EcoCyc (v14.0)

complete

GO:0000910

cytokinesis

PMID:9006034

IMP: Inferred from Mutant Phenotype

P

complete

GO:0007049

cell cycle

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001182

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

PMID:9006034

IMP: Inferred from Mutant Phenotype

P

complete

GO:0007049

cell cycle

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018365

P

Seeded from EcoCyc (v14.0)

complete

GO:0008360

regulation of cell shape

PMID:9006034

IMP: Inferred from Mutant Phenotype

P

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0032155

cell division site part

PMID:9603865

IDA: Inferred from Direct Assay

C

complete

GO:0008360

regulation of cell shape

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0133

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

PMID:9603865

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013437

P

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:18165305

IDA: Inferred from Direct Assay

F

FtsW was shown to interact with MtgA using a 2-hybrid system.

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0573

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001182

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013437

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018365

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:20497333

IPI: Inferred from Physical Interaction

EcoliWiki:ftsN


F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsP

PMID:19402753

LCMS(ID Probability):99.6

Protein

groS

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsT

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.6

Protein

phoA

PMID:19402753

LCMS(ID Probability):99.6

Protein

FtsI

FtsW activity in peptidoglycan synthesis at the septum is dependent on FtsI

PMID:11807049

Protein

FtsZ

ftsW201 is hypersensitive to excess levels of FtsZ at permissive temperatures

PMID:7961485


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 10 predicted transmembrane domains

Daley et al. (2005) [8]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRLSLPRLKM PRLPGFSILV WISTALKGWV MGSREKDTDS LIMYDRTLLW LTFGLAAIGF
IMVTSASMPI GQRLTNDPFF FAKRDGVYLI LAFILAIITL RLPMEFWQRY SATMLLGSII
LLMIVLVVGS SVKGASRWID LGLLRIQPAE LTKLSLFCYI ANYLVRKGDE VRNNLRGFLK
PMGVILVLAV LLLAQPDLGT VVVLFVTTLA MLFLAGAKLW QFIAIIGMGI SAVVLLILAE
PYRIRRVTAF WNPWEDPFGS GYQLTQSLMA FGRGELWGQG LGNSVQKLEY LPEAHTDFIF
AIIGEELGYV GVVLALLMVF FVAFRAMSIG RKALEIDHRF SGFLACSIGI WFSFQALVNV
GAAAGMLPTK GLTLPLISYG GSSLLIMSTA IMMLLRIDYE TRLEKAQAFV RGSR
Length

414

Mol. Wt

45.989 kDa

pI

10.2 (calculated)

Extinction coefficient

71,390 - 71,640 (calc based on 11 Y, 10 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

46..403

PF01098 Cell cycle protein

PMID:19920124

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<motif_map/>

tmhmm.php?gene=ftsW&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ftsW taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128082

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946322

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000320

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABG4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10344

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10344

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946322

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000338

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0340

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

293

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

117

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

169

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mraZW-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:98383..98423 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0089 (EcoliWiki Page)

NCBI GEO profiles for ftsW

microarray

GenExpDB:b0089 (EcoliWiki Page)

Summary of data for ftsW from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to ftsW Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10344

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0340

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0089

EcoGene

EcoGene:EG10344

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000338

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000320

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000038504 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

FTSW

From SHIGELLACYC

E. coli O157

FTSW

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01098 Cell cycle protein

EcoCyc

EcoCyc:EG10344

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10344

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000338

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0340

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000320

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Wang, L et al. (1998) FtsI and FtsW are localized to the septum in Escherichia coli. J. Bacteriol. 180 2810-6 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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